4个绵羊品种LHCGR基因单核苷酸多态性分析

doi:10.16431/j.cnki.1671-7236.2021.05.020

Abstract

Abstract: To explore the polymorphism and gene function of LHCGR gene of 4 sheep breeds,the 2 loci (g.75747421A>C and g.75748200T>A) of LHCGR gene among Dorper sheep,Tan sheep,Small-tailed Han sheep and Tan×Han hybrid sheep were detected by Sequenom MassARRAYSNP.The secondary structure of mRNA and the physicochemical properties,structure and protein interaction between the 2 sites before and after the mutation were predicted and analyzed by bioinformatics software.The results showed that there were 3 genotypes of LHCGR gene g.75747421A>C in 4 breeds,which were CC,CA and AA. g.75748200T>A had 3 genotypes of AA,AT and TT in 4 breeds.χ² test indicated that g.75747421A>C and g.75748200T>A were in Hardy-Weinberg equilibrium in 4 sheep breeds (P>0.05).Bioinformatics analysis showed that the relative molecular weight of LHCGR gene was 73 599.70,the theoretical isoelectric point was 8.30,the fat coefficient was 98.90,and the total average hydrophilicity was 0.191,which was an unstable hydrophobic protein.The secondary structure of LHCGR gene mRNA and the secondary structure and tertiary structure of the encoded protein were all changed before and after mutation.The results of protein-protein interaction showed that the proteins interacting with LHCGR protein mainly included bone morphogenetic protein 15 (BMP15), chromogranin A (CGA), mevalonate kinase (MVK), cholesterol side chain cleavage enzyme (CYP11A1), guanine nucleotide binding protein α subunit (GNAS).This study successfully screened the LHCGR gene polymorphism loci of 4 sheep breeds,which could provide a certain theoretical basis for the marker-assisted selection and breeding of sheep fecundity.

Key words: sheep; LHCGR gene; polymorphism; bioinformatics

CLC Number:

S813.3

SHAO Shuncheng, YAN Beibei, ZHANG Wen, ZHANG Tianwen, LIANG Peng, ZOU Shifan, ZHANG Kun, FENG Dengzhen. Single Nucleotide Polymorphism Analysis of LHCGR Gene in 4 Sheep Breeds[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1691-1698.

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Single Nucleotide Polymorphism Analysis of LHCGR Gene in 4 Sheep Breeds

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