TMEM219基因可变剪切体相对表达量及其对鹿茸重量的影响

doi:10.16431/j.cnki.1671-7236.2020.07.020

Abstract

Abstract: The purpose of the experiment was to study the expression patterns of three kinds of TMEM219 gene spliced antler at different weights and the effect of TMEM219 gene expression on the weight of velvet antler,in order to explore the regulatory mechanism of TMEM219 gene on the growth and development of velvet antler.The Real-time quantitative PCR technology was used to detect the relative expression levels of TMEM219 gene and its three spliced variants in different tissues of the same weight group and the same tissue of different weight groups of velvet antler.Simultaneously,the serum levels of insulin growth factor 1 (IGF-1) and insulin-like growth factor binding protein 3 (IGFBP-3) were measured and compared of Sika deer serum with different antler yields.The results showed that three kinds of spliceosome of TMEM219 gene were expressed in reserve mesenchyme and precartilage (RP),transition zone (TZ) and cartilage (C) of Sika deer antler.The relative expression of TMEM219-918 was extremely significantly higher than that of TMEM219-1005 and TMEM219-1960 (P<0.01),and there was no significant difference in the relative expression of TMEM219-1005 and TMEM219-1960 (P>0.05).The expression of TMEM219 gene in the high weight group was significantly higher than that in the low weight group (P<0.05).At the same time,the concentration of IGF-1 in the serum of high weight group was significantly higher than that of low weight group (P<0.05),while the IGFBP-3 concentration was significantly lower than that of low weight group (P<0.05).The results suggested that the high expression of TMEM219 gene might promote the growth of velvet antler and increase the weight of velvet antler.It was speculated that the possible mechanism was that TMEM219 competitively binded to IGFBP-3,reduced its IGF-1 binding ability,and strengthened the IGF-1 and IGF-1R affinity,which in turn enhanced the growth promotion effect of IGF-1 on velvet antler.TMEM219 gene would become a candidate gene that affected the growth and development of velvet antler,providing a theoretical basis for improving the growth of velvet antler.

Key words: velvet antler; TMEM219 gene; variable shear; fluorescence quantitative

CLC Number:

S825

ZHAO Pei, WANG Lei, WANG Hongliang, XING Xiumei, HU Pengfei. Relative Expression Level of TMEM219 Gene Variable Shear and Its Effect on Antler Weight[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2150-2158.

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Relative Expression Level of TMEM219 Gene Variable Shear and Its Effect on Antler Weight

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