豫西脂尾羊INSIG1基因克隆、序列分析及组织表达

doi:10.16431/j.cnki.1671-7236.2020.06.017

Abstract

Abstract: Insulin induced gene 1 (INSIG1) was an important regulatory gene of lipid synthesis and lipolysis.This study was aimed to clone INSIG1 gene,analyze the sequences by bioinformatics software,and measure the expression of INSIG1 gene in different tissues of Yuxi Fat-tailed sheep.The tissue RNA was extracted by Trizol method,and the INSIG1 gene sequence was obtained by RT-PCR and cloning.The expression of INSIG1 mRNA was detected by Real-time PCR.The results showed that the complete CDS of sheep INSIG1 gene was successfully cloned which was 831 bp and encoded 276 amino acids.The homology analysis showed that the INSIG1 coding region in Yuxi Fat-tailed sheep was 99.64% similar to that of sheep (XM_015095466.2),and the similarity of coding amino acid sequence was 99.28%.The physical and chemical properties of INSIG1 protein showed that the molecular weight was 29.58 ku and the theoretical isoelectric point (pI) was 9.07,which belonged to stable alkaline hydrophobic protein.There was 5 transmembrane structures and no signal peptide in INSIG1 protein, mainly located in cytoplasm.The tertiary structure of INSIG1 protein mainly consisted of 6 alpha helices and random coils.Real-time PCR results showed that the expression of INSIG1 gene in liver was obviously higher than other tissues,which followed by lung,intestine,and adipose of tail,and the lowest was in muscle.This study improved the database of Yuxi Fat-tailed sheep,and provided basic data for further study on the function of INSIG1 gene in fat deposition and regulation of mutton quality in sheep.

Key words: Yuxi Fat-tailed sheep; INSIG1 gene; cloning; tissue expression

CLC Number:

S826

QUAN Kai, SONG Feihu, LI Jun. Cloning,Sequence Analysis and Tissue Expression of INSIG1 Gene in Yuxi Fat-tailed Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1781-1790.

References

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Cloning,Sequence Analysis and Tissue Expression of INSIG1 Gene in Yuxi Fat-tailed Sheep

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