促卵泡素对体外培养牛卵泡类固醇合成相关基因表达的影响

doi:10.16431/j.cnki.1671-7236.2019.11.019

Abstract

Abstract: The purpose of this study was to investigate the effects of FSH treatment on steroidogenesis genes expression in granulosa cells and theca cells of cultured bovine antral follicles in vitro.Cavernous follicles 9-11 mm in diameter on the surface of bovine ovaries were collected and cultured in vitro with DMEM/F12 containing different concentrations of FSH for 24 hours.The total RNA of granulosa cells and theca cells were extracted,and reverse transcribed into cDNA,the expression of steroidogenesis genes (CYP11A1,3β-HSD,CYP17A1,CYP19A1 and 17β-HSD) and gonadotropin receptor genes (FSHR and LHR) in follicular granulosa cells and theca cells were detected by Real-time quantitative PCR.The results in granulosa cells showed that FSH up-regulated the expression of CYP11A1,3β-HSD and CYP19A1 genes,25 ng/mL FSH treatment extremely significantly increased the expression of CYP11A1 gene (P<0.01),10 ng/mL FSH treatment significantly increased the expression of 3β-HSD gene (P<0.05),50 ng/mL FSH treatment significantly increased the expression of CYP19A1 gene (P<0.05).The results in theca cells showed that 50 ng/mL FSH treatment significantly or extremely significantly increased the expression of CYP11A1,3β-HSD and CYP17A1 genes (P<0.05;P<0.01),but 10 and 25 ng/mL FSH treatment significantly or extremely significantly decreased the expression of CYP11A1,3β-HSD and CYP17A1 genes (P<0.05;P<0.01).The results showed that FSH treatment had no significant effect on the expression of FSHR and LHR genes in granulosa cells (P>0.05),only 25 and 50 ng/mL FSH treatments significantly or extremely significantly increased the expression of LHR gene in theca cells (P<0.05;P<0.01).The expression of CYP11A1,3β-HSD and CYP17A1 genes in theca cells among the different treatment groups showed the same trend as that of LHR gene.The results indicated that FSH treatment could increase the expression of CYP11A1,3β-HSD and CYP17A1 genes in granulosa cells of bovine antral follicular,the expression of CYP11A1,3β-HSD and CYP17A1 genes in theca cells were more sensitive to the stimulation of LH.FSH might regulate the expression of steroidogenesis genes in theca cells by affecting the expression of LHR gene.

Key words: bovine; FSH; antral follicles; steroidogenesis genes; in vitro test

CLC Number:

S814.8

WANG Yangyang, XU Huitao, ZHAO Shanjiang, PANG Yunwei, HAO Haisheng, ZOU Huiying, ZHU Huabin. Effects of FSH on Genes Expression Related to Steroidogenesis in Bovine Follicles Cultured in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3299-3306.

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Effects of FSH on Genes Expression Related to Steroidogenesis in Bovine Follicles Cultured in vitro

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