牛源IL-6基因克隆与生物信息学分析

doi:10.16431/j.cnki.1671-7236.2019.11.004

Abstract

Abstract: The aim of the experiment was to clone the bovine interleukin-6 (IL-6) gene and analyze its coding protein bioinformatically.RNA was extracted from the tail heel blood of Holstein cows and retrieved into the cDNA.Specific primers were designed according to the known bovine IL-6 gene sequence in NCBI database,the full-length sequence of CDS region was cloned by RT-PCR.The biological characteristics of bovine IL-6 gene sequence,homology and physicochemical properties of coding products were analyzed by bioinformatics software.The results showed that the bovine IL-6 gene CDS region was successfully cloned,the total length was 627 bp,and the molecular weight was 23.759 ku,encoding a total of 208 amino acids.The theoretical isoelectric point was 7.58,the fat soluble coefficient was 93.37,which belonged to the hydrophilic protein.The homologies of Bos taurus IL-6 gene with Sus scrofa,Ovis aries,Rattus norvegicus,Homo sapiens,Felis catus,Canis lupus familiaris,Gallus gallus,Mus musculus,Equus caballus and Oryctolagus cuniculus were 84.4%,96.2%,66.7%,77.5%,75.9%,79.3%,48.5%,67.0%,79.5% and 67.5%,respectively.Phylogenetic tree analysis revealed that cattle and sheep had the closest genetic relationship,followed by pigs,and chickens was farthest.In the secondary structure prediction,the random coil and alpha-helix of the protein were 34.6% and 60.1% respectively.Subcellular localization was located in cytoplasm;The protein contained signal peptide and transmembrane helix structure.This study laid a theoretical foundation for further analysis of bovine IL-6 gene function.

Key words: bovine; IL-6 gene; cloning; bioinformatics analysis

CLC Number:

S858.23

LYU Yanan, LIU Jingyu, YAO Jingjie, AN Zhixing, BAI Yueyu, ZHANG Xiaojian. Cloning and Bioinformatics Analysis of IL-6 Gene in Bovine[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3163-3171.

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Cloning and Bioinformatics Analysis of IL-6 Gene in Bovine

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