牦牛Myf6基因克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2019.06.005

Abstract

Abstract:

The aim of this study was to clone and analyze Myf6 gene in yaks by bioinformatics,and investigate its expression in different periods.The snowy yak in Qinghai was taken as the research object.The Myf6 gene CDS region was cloned and sequenced by designing primers,and its functional structure was predicted by bioinformatics software.The results showed that the yak Myf6 gene contained a 729 bp open reading frame (ORF) encoding 242 amino acids.The results of homology alignment and phylogenetic tree analysis indicated that the amino acid sequence of yak Myf6 was close to common cattle and sheep.The physicochemical properties of Myf6 protein were predicted and analyzed.The structural formula of Myf6 protein was C₁₁₆₇H₁₈₇₀N₃₃₆O₃₇₂S₁₃,and the theoretical isoelectric point was 5.64,with serine being the highest,followed by leucine,glutamic acid,proline and arginine.The hydrophobic average was -0.586,the most hydrophilic was -2.467,and the hydrophobicity was 1.511.It was predicted that the yak Myf6 was predicted to be hydrophilic and soluble protein.There were portions that could form coiled coil;The phosphorylation sites results showed that there were 26 serine kinases,10 threonine kinases and 5 tyrosine kinases;Subcellular localization analysis showed that the encoded product was most distributed in the nucleus (73.9%),followed by cytoskeleton (13.0%),cytoplasm (4.3%),Golgi apparatus (4.3%) and secretory system vesicles (4.3%).The protein secondary structure was predicted to consist mainly of random coils (49.17%),followed by alpha helix (33.47%),extended chain (11.57%) and beta-sheet (5.79%).Functional analysis of the protein showed that the growth factor function had a higher probability (7.694),followed by transcriptional regulation,which was predicted to participate in the biological regulation of the body as a growth factor.The results of Real-time PCR showed that Myf6 gene was expressed in the longissimus dorsi muscle of fetal calf,6 months old and 4 to 5 years old,and the expression level at 6 months old was significantly higher than that of fetal cattle and 4 to 5 years old (P<0.05).The above results provided important research data for the study of yak Myf6 myogenic factors,and provided a reference for improving the economic traits of yak.

Key words: yak; Myf6 gene; bioinformatics analysis; cloning

CLC Number:

S823.8⁺5

LEI Qinyi, FU Donghai, CHANG Yongfang, WU Xiaoyun, LIANG Chunnian, CHU Min, YAN Ping. Cloning and Bioinformatics Analysis of Myf6 Gene in Yak[J]. , 2019, 46(6): 1603-1611.

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Cloning and Bioinformatics Analysis of Myf6 Gene in Yak

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