豫西脂尾羊CIDEa基因的克隆、序列分析与组织表达

doi:10.16431/j.cnki.1671-7236.2019.06.004

Abstract

Abstract:

This study was aimed to clone cell death-inducing DNA fragmentation factor 45-like effector A (CIDEa),analyze the sequences by bioinformatics software,and detect the expression of CIDEa gene in different tissues of Yuxi fat-tailed sheep.Using RNA of the adipose tissue in Yuxi fat-tailed sheep as a template,the complete CDS sequence of CIDEa gene in Yuxi fat-tailed sheep was amplified and cloned,and the sequencing result was analyzed by bioinformatics.The expression of CIDEa gene in heart,liver,lung,spleen,kidney,rumen,small intestine,longissimus dorsal muscle,subcutaneous adipose and visceral adipose tissue of Yuxi fat-tailed sheep were analyzed.The results showed that the length of CDS sequence of CIDEa gene in Yuxi fat-tailed sheep was 660 bp and encoded 219 amino acids.The homology of CIDEa gene in Yuxi fat-tailed sheep were 99.1%,96.8%,96.2%,98.8%,85.0% and 79.4% with Ovis aries,Bubalus bubalis,Bos taurus,Pantholops hodgsonii,Sus scrofa and Homo sapiens,respectively.The physical and chemical properties of CIDEa protein showed that the molecular weight was 24.38 ku and the theoretical isoelectric point (pI) was 9.12,which belonged to alkaline protein.There was no transmembrane structure and signal peptide in CIDEa protein.The subcellular localization results showed that CIDEa protein was located in the cytoplasm (47.8%),mitochondria (26.1%),nuclear (17.4%),vacuolar (4.3%) and endoplasmic reticulum (4.3%).The tertiary structure of CIDEa protein mainly consisted of 2 alpha helices,4 beta foldings and random coils.Real-time quantitative PCR results showed that the expression of CIDEa gene in subcutaneous adipose and visceral adipose were obviously higher than other tissues,which followed by intestine,liver,spleen,rumen,heart,kidney,lung and longissimus dorsi muscle ranged from high to low.This results provided basic data for further study on the function of CIDEa gene in lipid metabolism and regulation of mutton quality in sheep.

Key words: Yuxi fat-tailed sheep; CIDEagene; cloning; bioinformatics analysis; tissue expression

CLC Number:

LI Jun, HOU Xiafei, LIANG Wenshuang, DENG Hongyu, ZHANG Guizhi, LIU Taiyu, WANG Xiaoxiao, QUAN Kai. Cloning,Sequence Analysis and Tissue Expression of CIDEa Gene in Yuxi Fat-tailed Sheep[J]. , 2019, 46(6): 1594-1602.

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Cloning,Sequence Analysis and Tissue Expression of CIDEa Gene in Yuxi Fat-tailed Sheep

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