猪黄体不同发育时期活性氧水平及多种抗氧化酶mRNA表达量变化规律的研究

doi:10.16431/j.cnki.1671-7236.2019.03.021

Abstract

Abstract:

The purpose of the experiment was to study the change law of reactive oxygen species (ROS) and the expression of antioxidant enzymes genes in the development and degradation of porcine corpus luteum,and to supplement the theoretical basis and provide new ideas for interpreting the antioxidant mechanism of porcine corpus luteum.The ovaries used in the experiment were collected from Yanji slaughterhouse.After removed from the ovary,the corpus luteum was initially divided into early corpus luteum,middle corpus luteum,late corpus luteum and white bodies by its size.The middle and late corpus luteum were then accurately separated by detecting progesterone levels.The levels of ROS in the corpus luteum were detected by frozen section and DHE fluorescence staining technique.The amount of expression of manganese superoxide dismutase (Mn-SOD),catalase (CAT) and Glutathione peroxidase (GPx) of antioxidant enzymes were examined by Real-time PCR in each period.The results showed that the ROS level of porcine corpus luteum increased regularly with the periodic development of porcine corpus luteum,that was,ROS level in the late period were significantly higher than that in other periods (P<0.05).And the ROS level in the middle period was significantly higher than that in the initial period and white body (P<0.05),and there was no significant difference between white body and the initial period (P>0.05).The expression of Mn-SOD, GPx1 and GPx4 decreased regularly,they were significantly higher in the initial period and white body than that in the middle and late stage (P<0.05),and their expression in the middle stage was significantly higher than that in the late stage (P<0.05).and the white body was significantly higher than that in other stages (P<0.05).The expression of GPx3 gene in white body was significantly higher than that in other stages (P<0.05),the expression in initial period was significantly higher than that in the middle and later stage (P<0.05),and the expression in the middle stage was significantly higher than that in the late stage (P<0.05).The CAT expression had no significant change with the development of the corpus luteum (P>0.05).In conclusion,ROS,Mn-SOD and GPx were the influencing factors for the development and degradation of corpus luteum,and the level of ROS was opposite to that of Mn-SOD and GPx.

Key words: sow; corpusluteum; ROS; Mn-SOD; GPx

CLC Number:

S821.3

GAO Jianhong, LI Chuang, QIAO Jiaqi, FANG Nanzhu, LI Zhongshu, LIU Haixing. Study on the Change Law of Reactive Oxygen Level and the Expression of Various Antioxidant Enzymes mRNA in Different Developmental Stages of Porcine Corpus Luteum[J]. , 2019, 46(3): 818-823.

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Study on the Change Law of Reactive Oxygen Level and the Expression of Various Antioxidant Enzymes mRNA in Different Developmental Stages of Porcine Corpus Luteum

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