无菌大鼠深二度烫伤感染模型机体炎性反应的研究

doi:10.16431/j.cnki.1671-7236.2018.12.038

Abstract

Abstract:

To study the inflammatory response of germ-free rats with deep second-degree scald injury,a deep second-degree scald injury model of germ-free rats which were infected with Pseudomonas aeruginosa and a deep second-degree scald injury model of SPF rats were established,then the similarities and differences between the three groups in terms of healing process,inflammatory response,and pathological changes were compared,and the effect of inflammatory reaction of deep second-degree scald injury sterile rats were explored.Deep second degree scald wound was performed in 20 6-week-old female germ-free rats at the temperature of 94℃,8 s in a clean bench.And then the scald germ-free rats were randomly divided into two groups,one group was infected with Pseudomonas aeruginosa with a concentration of 1×10⁵ CFU/mL in the wound,the other group was not infected and the wound kept clean.SPF scald Wistar rats were set up as control group under the same conditions.The scab escharosis time,dislocation time and healing time of the three groups were observed,and serum samples were taken to detect the changes of inflammatory cytokines such as TNF-α,IL-1α and GM-CSF before burned (0 h) and 24 h,3 d and 7 d after burned.The results showed that the Pseudomonas aeruginosa infection rats had a long period of scab escharosis time (P<0.05) and the decrustation and healing time was significantly short compared with SPF rat and germ-free rats (P<0.05).When the burned rats were infected with Pseudomonas aeruginosa for 24 h,lymphocytes were observed in the subcutaneous tissues and there were chronic inflammatory reactions.At the 3rd day,there was a purulent infection in the deep dermis,and a large number of inflammatory cells were seen around the striated muscle.On the 7th day,there was slight blood vessel dilatation and congestion,and purulent infection was relieved.The skin of germ-free scald rats and SPF burned rats had mild inflammatory reactions within one week after scald.The serum level of TNF-α,GM-CSF and IL-1α in three groups of rats were significantly increased after burned (P<0.05);At 24 h,the level of IL-1α in the germ-free scald rats and the scald rats infected with Pseudomonas aeruginosa were significantly higher than the SPF rats (P<0.05),while the GM-CSF content in germ-free scald rats was significantly higher than that in the scald rats infected with Pseudomonas aeruginosa and SPF rats (P<0.05).At the 3rd day,the IL-1α content in germ-free scald rats and the scald rats infected with Pseudomonas aeruginosa were significantly higher than the SPF rats (P<0.05),and the GM-CSF content in germ-free scald rats was significantly higher than that in the scald rats infected with Pseudomonas aeruginosa (P<0.05).In conclusion,germ-free scald rats reacted fiercely,and serum inflammatory factors increased rapidly,while the inflammatory response of SPF rats was slower to the deep second degree scald.Pseudomonas aeruginosa infected deep second-degree scald sterile rats had a shorter decrustation time and healing time.

Key words: germ-free rat; Pseudomonas aeruginosa; scald; inflammatory reaction

CLC Number:

R644

LI Hongyan, YAN Kesong, MA Dongsheng, ZHAN Dawei, SUN Zhaozeng. Study on Inflammatory Response of Germ-free Rats with Second Degree Scald[J]. , 2018, 45(12): 3623-3629.

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Study on Inflammatory Response of Germ-free Rats with Second Degree Scald

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