结核分枝杆菌PhoP-PhoR双组分系统研究进展

doi:10.16431/j.cnki.1671-7236.2018.11.033

Abstract

Abstract:

The two-component signal transduction system is widely distributed in various prokaryotes.Its basic structure consist of a histidine protein kinase (HK) and a response-regulatory protein (RR).The system is essential for the virulence and growth of bacteria which can perceive various external stimuli and react,so that bacteria can adapt to various adverse conditions and enhance the survival ability in the host.In this paper,12 two-component systems of Mycobacterium tuberculosis including DosS/DosT-DosR,MprA-MprB,PrrA-PrrB and PhoP-PhoR are briefly reviewed.The structure and function of PhoP-PhoR are highlighted.PhoP-PhoR is one of the most basic and most important two component systems of Mycobacterium tuberculosis,composed of receptor PhoR and effector PhoP,in which PhoR can receive external stimuli such as Mg²⁺,Cl^- or H⁺,and then drive PhoP to regulate the transcriptional expression of target genes.As an effector protein,PhoP can be found through the analysis of crystal structure,which can be used to regulate the target gene by combining with DNA,including the control of cell wall composition,the regulation of lipid metabolism and pH,and the regulation of the virulence network of Mycobacterium tuberculosis.In addition,the advantages of PhoP mutant strain as a vaccine candidate is also introduced in the article,including the weakening of PhoP mutant strain in the mouse model,and immune protection in immunized Ganges River monkeys and guinea pigs,indicating that the PhoP mutant has a good potential for vaccine development.In this paper,the structure and function of PhoP-PhoR two-component system and the research of PhoP mutant as vaccine candidate are reviewed to provide a theoretical basis for research of the virulence mechanism of Mycobacterium tuberculosis and human tuberculosis vaccine.

Key words: Mycobacterium tuberculosis; PhoP-PhoR two-component system; PhoP protein; PhoP mutant

CLC Number:

S852.61⁺8

GUO Jiacheng, REN Ningning, GUO Aizhen, CHEN Yingyu. Research Progress on Two-component System of Mycobacterium tuberculosis PhoP-PhoR[J]. , 2018, 45(11): 3253-3260.

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Research Progress on Two-component System of Mycobacterium tuberculosis PhoP-PhoR

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