鹿结核分枝杆菌MPB70蛋白的表达与免疫原性鉴定

doi:10.16431/j.cnki.1671-7236.2016.12.008

Abstract

Abstract:

In this study,the immunogenicity protein MPB70 gene was amplified from Mycobacterium tuberculosis genome DNA which separated from deer, and about 590 bp fragment was obtained. Then the fragment was cloned and constructed prokaryotic expression vector of pET-30a-MPB70, and the recombinant plasmid was put into E. coli BL21(DE3).Purified after IPTG induction, and analyzed by SDS-PAGE, a specificity protein band was observed at 20 ku. Using the deer serum positive of tuberculosis in Western blotting, the fusion protein could be combined with deer serum positive of tuberculosis antibody and arise specific immune response. The protein could be used as a specific antigen to test the deer tuberculosis. The study laid a foundation for further studying the deer tuberculosis appraisal method.

Key words: Mycobacterium tuberculosis; MPB70 gene; prokaryotic expression

CLC Number:

S852.61⁺8

WANG Xiao-long, LU Tian-cheng, WANG Xiu-ran, YANG Yan-ling. Expression of MPB70 Protein and Identification the Immunogenicity of Deer Mycobacterium tuberculosis[J]. , 2016, 43(12): 3135-3140.

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Expression of MPB70 Protein and Identification the Immunogenicity of Deer Mycobacterium tuberculosis

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