奶山羊LF基因CDS区克隆、生物信息学分析及其乳中含量测定

doi:10.16431/j.cnki.1671-7236.2018.10.017

Abstract

Abstract:

In order to explore the biological characteristics of lactoferrin (LF) gene in dairy goat,and analyze the variations of LF of milk in Saanen dairy goats,CDS region of LF gene in Saanen dairy goat was cloned,the sequence,physicochemical property,conservative structure domain and homology comparison of LF gene were analyzed by bioinformatics softwares,milk samples from individual were obtained and determined using ELISA kit.The results showed that the length of LF gene in Saanen dairy goat was 2 127 bp,which coded 708 amino acids.The formula of LF protein was C₃₄₀₅H₅₃₆₅N₉₄₉O₁₀₂₄S₄₃,which was an unstable and hydrophilic protein.The secondary structure of LF mainly contained α-helix,extended chain and random coil,similar with that in transferrin.The molecular weight of LF was 77.359 ku.The protein sequence analysis result showed that the high similarity were found in Bovidae (> 92%),and the rest were quite different,LF gene of Saanen dairy goat exhibited the highest homology with goat,they shared 99% amino acid identity and were in the same branch of an evolutionary tree.ELISA results showed that the maximal concentrations were observed in the colostral samples,the common milk had the lower and stable concentrations.The results obtained the sequence and molecular structure of LF gene,and revealed the change of LF content in milk of Saanen dairy goats,which provided a theoretical basis for LF functional researches and product development.

Key words: Saanen dairy goat; LF gene; cloning; bioinformatics

CLC Number:

WANG Jianjue, SHI Huaiping, MA Gongzhen, HUANG Jiangtao, LV Ming, ZHANG Zhifei. Cloning and Bioinformatics Analysis of LF Gene CDS Region and Its Content Determination of Milk in Dairy Goats[J]. , 2018, 45(10): 2805-2813.

References

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Cloning and Bioinformatics Analysis of LF Gene CDS Region and Its Content Determination of Milk in Dairy Goats

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