伏牛白山羊TACR1基因扩增及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2018.09.008

Abstract

Abstract:

The purpose of this study was to clone the TACR1 gene of Funiu White goat and analyze its structure and encoded protein by bioinformatics methods to lay a foundation for further study on the function of TACR1 gene and its role in reproductive physiology and immune regulation.5 pairs of primers were designed based on the goat TACR1 gene sequence in GenBank (accession No:NC_030818.1).The Funiu White goat TACR1 gene was amplified by PCR amplification and sequenced,and the complete coding region (CDS) of TACR1 gene was obtained after splicing the fragments.The results showed that the open reading frame(CRF) of TACR1 gene in Funiu White goat was 852 bp in length and encoded a total of 283 amino acids.The content of base composition was A (23.47%),T (25.49%),C (27.76%) and G (23.27%).Compared with sheep TACR1 gene, the TACR1 gene CDS region of the Funiu White goat had a two-base mutation which did not cause amino acid changes.The non-coding region at the 5'and 3' ends was 860 and 271 bp in length, and had 9 and 2 base mutations,respectively.The results of homology analysis showed that the identity of TACR1 gene between Funiu White goat and Capra hircus,Pantholops hodgsonii,Ovis aries,Bos indicus,Odocoileus virginianus,Lipotes vexillifer,Homo sapiens,Sus scrofa,Panthera pardus,Cavia porcellus,Equus asinus were 99.9%,99.3%,99.2%,97.5%,96.7%,92.9%,89.7%,88.6%, 86.3%,86.2% and 85.0%,respectively.There was a signal peptide at the N-terminus of TACR1 and there might be a cleavage site at 21th amino acid predicted by SignalP4.1 online software.Through the prediction of protein structure,it was found that the protein encoded by TACR1 gene of the FuNiu White goat had no transmembrane domain.

Key words: Funiu White goat; TACR1 gene; bioinformatics analysis

CLC Number:

Q785

SHI Huibin, WANG Yuqin, TIAN Zhilong, ZAHNG Xiaohui, WANG Jianping, YANG Fang, LI Yuanxiao. Amplification and Bioinformatics Analysis of TACR1 Gene in Funiu White Goat[J]. , 2018, 45(9): 2409-2416.

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Amplification and Bioinformatics Analysis of TACR1 Gene in Funiu White Goat

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