醋酸诱导犬急性溃疡性结肠炎模型的建立

doi:10.16431/j.cnki.1671-7236.2018.05.034

Abstract

Abstract:

The study was aimed to establish a stable model of acute ulcerative colitis (UC) in dogs using acetic acid.Sixteen healthy dogs were randomly divided into four groups:Control group and three acetic acid (with different concentrations) groups.After anesthetic Quanmianbao injection,the acetic acid groups was perfused with 4%,7% and 10% 2 mL/kg acetic acid,respectively.The control group was not anesthetized and the rectal perfusion was given the same dose of sterile saline.The defecation and the colorectal mucosa were observed by electronic endoscopy at the 1st,3rd and 7th days after injection.All the experimental dogs were sacrificed on the 7th day.The lesioned tissues were collected and examined by histological.The results showed that:In 4% acetic acid group,short-term dilute feces and mild inflammatory cells infiltration was observed.In 10% acetic acid group,dogs died because of strong inflammation which lead to colon perforation.In 7% acetic acid group,both fecal traits and colon endoscopy check showed significant symptoms of colitis,and ulcer foci formed and droped off after 3 and 7 d of acetic acid administration,respectively.Histological examination showed that there was significant inflammatory cells infiltration around the ulcer foci,and it would significantly reduced as time goes on,and replaced by fibroblast hyperplasia.In conclusion,acute ulcerative colitis in dogs could be successfully induced by 7% acetic acid perfusion.

Key words: acetic acid; dog; ulcerative colitis (UC)

CLC Number:

S854

CHEN Li, XU Zaipin, ZHANG Chenggui, WU Xiumei, XU Yulan, CHEN Guang, DU Jianbin. Establishment of Acute Ulcerative Colitis Model Induced by Acetic Acid in Dog[J]. , 2018, 45(5): 1395-1400.

References

[1] 王新月,田德禄.溃疡性结肠炎病因病理特点与中医辨治思路对策[J].北京中医药大学学报,2007,8:554-555. WANG X Y,TIAN D L.Etiological and pathological characteristics of ulcerative colitis and TCM treatment strategies[J]. Journal of Beijing University of Chinese Medicine,2007,8:554-555.(in Chinese)
[2] 黎人铚,张俊,张杰伟,等.中药内服加灌肠治疗活动期溃疡性结肠炎33例[J].中国实验方剂学杂志,2012,18(2):233-234. LI R Z,ZHANG J,ZHANG J W,et al.Chinese medicine plus enemaactive treatment of ulcerative colitis in 33 cases[J]. Chinese Journal of Experimental Traditional Medical Fomulae,2012,18(2):233-234.(in Chinese)
[3] RUTTER M D,SANUDERS B P,WILKINSON K H,et al.Severity of inflammation is a risk factor for colorectal neoplasia in ulcerative colitis[J].World Journal of Gastroenterology,2004,126:451-459.
[4] 钱家鸣,杨红.中国炎症性肠病研究的历史回顾现状和展望[J].中国实用内科杂志,2015,35(9):727-730. QIAN J M,YANG H.History,current situation and progress of inflammatory bowel disease in China[J].Chinese Journal of Practical Internal Medicine,2015,35(9):727-730.(in Chinese)
[5] JOSEPH D,ADAM S.Ulcerative colitis epidemiology,diagnosis,and management[J].Mayo Clinc Proceedings,2014,89(11):1553-1563.
[6] PLEVY S,SILVERBERG M S,LOCKTON S,et al.Combinedserological,genetic,and inflammatory markers differentiate Non-IBD,crohn's disease,and ulcerative colitis patients[J].Inflamm Bowel Diseases,2013,19(6):1139-1148.
[7] SCIOLI M G,STASI M A,PASSERI D,et al.Propionyl-lcarnitineis efficacious in ulcerative colitis through its action on the immune function and microvasculature[J]. Clinical and Translation Gastroenterology,2014,5(3):55.
[8] 郑丽红,王海强.中医药治疗溃疡性结肠炎思路探索[J].光明中医,2014,29(7):1568-1569. ZHENG L H,WANG H Q.Chinese medicine treatment of ulcerative colitis thinking[J].Bright Chinese Medicine,2014,29(7):1568-1569.(in Chinese)
[9] LEGAHI E,GAZOULI M.Influence of environmental factors in the development of inflammatory bowel diseases[J].World Journal of Gastrointestinal Pharmacology and Therapeutics,2016,7(1):112-125.
[10] 曹明泽,王旭荣,王磊,等.小鼠溃疡性结肠炎模型的建立与评价[J].中国畜牧兽医, 2016, 43(1):171-175. CAO M Z,WANG X R,WANG L,et al.Establishment and evaluation of mouse model of ulcerative[J].China Animal Husbandry & Veterinary Medicine,2016,43(1):171-175.(in Chinese)
[11] 于丰彦,古文莉,罗琦,等.周福生教授治疗溃疡性结肠炎经验总结[A].第二十次全国中西医结合消化系统疾病学术会议暨消化疾病诊治进展[C].上海:2008. YU F Y,GU W L,LUO Q,et al.Professor Zhou Fusheng's experience in the treatment of ulcerative colitis[A].20th National Symposium on Integrative Digestive System Diseases and Progress in Diagnosis and Treatment of Digestive Diseases[C].Shanghai:2008.(in Chinese)
[12] HANG Z L,FAN H Y,YANG M Y,et al.Therapeutic effect of a hydroxynaphthoquinone fraction on dextran sulfate sodium-induced ulcerative colitis[J].World Journal of Gastroenterology,2014,20(41):15310-15318.
[13] 王朋川,何苗,张汉超,等.康复新液对2,4-二硝基氯苯联合醋酸诱导大鼠溃疡性结肠炎的影响[J].大理大学学报,2016,1(4):17-21. WANG M C,HE M,ZHANG H C,et al.Effect of Kangfuxin solution to ulcerative colitis rat model made by treatment of 2,4-dinitrochlorobenzene combined with acetic acid[J].Journal of DaLi University,2016,1(4):17-21.(in Chinese)
[14] FABIA R,WILEN R,AR RAJAB A,et al.Acetic acid-induced colitis in the rat:A reproducible experimental model for acute ulcerative colitis[J].European Surgical Research,1992,24(4):211-225.
[15] 吕宾.肠黏膜屏障与肠功能障碍[J].现代消化及介入诊疗,2013,18(4):232-234. LV B.Intestinal mucosal barrier and intestinal dysfunction[J].Journal of Modern Digestion and Interventional Diagnosis,2013,18(4):232-234.(in Chinese)
[16] 于艳.苏木乙酸乙酯提取物对溃疡性结肠炎大鼠T细胞亚群和IL-6的影响[J].中国畜牧兽医,2010,37(12):45-47. YU Y.Effect of ethyl acetate extract from sappan wood on T cells subsets[J].China Animal Husbandry & Veterinary Medicine,2010,37(12):45-47.(in Chinese)
[17] THIPPESWAMY B S,MAHENDRAN S,BIRADAR M I,et al.Protective effect of embelin against acid induced ulcerative colitis in rats[J].European Journal of Pharmacology,2011,654(1):100-104.
[18] HOLMA R,SALMENPERA P,RIUTTA A,et al.Acute effects of the cys-leukotriene-1 receptor antagonist,montelukast on experimental colitis in rats[J].European Journal of Pharmacology,2001,429(1-3):309-318.
[19] 郑文潇,吴翔.溃疡性结肠炎动物模型的研究进展[J].中国病原生物学杂志.2016,11(9):861-864. ZHENG W X,WU X.A review animal models of ulcerative colitis[J]. Journal of Pathogen Biology,2016,11(9):861-864.(in Chinese)
[20] 张月凡.溃疡性结肠炎患者组织差异蛋白质组学初步研究及其急性损伤动物模型的建立[D].北京:中国人民解放军军医进修学院,2012. ZHANG Y F.Differential proteomic analysis of tissues from ulcerative colitis patients and the establishment of acute damaged models with ulcerative colitis[D].Beijing:Chinese People's Liberation Army Academy of Military Medical Training,2012.(in Chinese)
[21] 时松,黄振,罗熠.白芨多糖对小鼠溃疡性结肠炎的治疗作用[J].中国药科大学学报,2012,43(6):535-540. SHI S,HUANG Z,LUO Y.Bletilla striata polysaccharide for the treatment of ulcerative colitis in mice[J].Journal of China Pharmaceutical University,2012,43(6):535-540.(in Chinese)
[22] DAVOOD M,FARANAK B,SEVED V H,et al.The healing effect of teucrium polium in acetic acid-induced ulcerative colitis in the dog as an animal model[J].Middle East Journal of Digestive Diseases,2012,4(1):40-44.
[23] 杜力军,赵玉男.实验动物与实验动物模型[M].北京:中国医药科技出版社,2012. DU L J,ZHAO Y N.Experimental Animal and Experimental Animal Model[M].Beijing:China Medical Science and Technology Press,2012.(in Chinese)
[24] 刘亚,李兴广.常用溃疡性结肠炎动物模型的探讨及比较[J].价值工程,2012,31(1):290-291. LIU Y,LI X G.Exploring and comparing animal models of common ulcerative colitis[J].Value Engineering,2012,31(1):290-291.(in Chinese)
[25] 陈星,王清静,王蕾,等.仔猪溃疡性结肠炎模型的建立[J].饲料工业,2013,34(19):24-26. CHEN X,WANG Q J,WANG L,et al.Establishment of a piglet ulcerative colitis model[J].Feed Industry,2013,13(19):24-26.(in Chinese)

Establishment of Acute Ulcerative Colitis Model Induced by Acetic Acid in Dog

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