巨型玫瑰冠鸡H-FABP、A-FABP基因表达的发育性变化及其肌内脂肪含量研究

doi:10.16431/j.cnki.1671-7236.2018.05.021

Abstract

Abstract:

In order to investigate the relationship between heart-type fatty acid-binding protein (H-FABP) and adipocyte fatty acid-binding protein (A-FABP) genes and the growth and intramuscular fat (IMF) content of Rose cockscomb chicken,and the mechanism of H-FABP and A-FABP genes effects on the IMF content and abdominal fat deposition,a total of 480 copies of the abdominal fat, myocardium,chest muscle and leg muscle samples of Rose cockscomb and Liangfenghua chickens were collected,the H-FABP and A-FABP genes mRNA expression were analyzed by Real-time quantitative PCR,and the IMF content of chest and leg muscles of 12-week-old chickens was determined by Soxhlet extraction method.The results showed that the H-FABP and A-FABP genes were all expressed in abdominal fat, myocardium,chest muscle and leg muscle tissues with varying degrees.The H-FABP genes of Rose cockscomb and Liangfenghua chickens was highly expressed in myocardium tissues,moderately expressed in chest and leg muscles tissues,and rarely expressed in abdominal fat tissues.And the expression of A-FABP gene was on the contrary,indicating that H-FABP gene was mainly expressed in muscle tissues while A-FABP gene was mainly expressed in adipose tissue.Liangfenghua chicken grew faster than Rose cockscomb chicken,the abdominal fat rat of Rose cockscomb chicken were lower than single-sex Liangfenghua chicken,but the IMF in chest muscle and leg muscle were higher than that of single-sex Liangfenghua chicken,showing that the meat quality of Rose cockscomb chicken was better.The test result laid a solid foundation for the H-FABP and A-FABP genes molecular breeding of Rose cockscomb chicken.

Key words: Rose cockscomb chicken; Lianfenghua chicken; H-FABP gene; A-FABP gene; IMF

CLC Number:

S813.2

ZHOU Zhinan, MAO Haipeng, DING Qiuyun, WU Changchi, FENG Zhenhua, ZHANG Mei, ZHANG Miaomiao, SUN Jie, LIAO Herong. The Developmental Changes of H-FABP and A-FABP Genes Expression and Intramuscular Fat Content of Rose Cockscomb Chicken[J]. , 2018, 45(5): 1289-1296.

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The Developmental Changes of H-FABP and A-FABP Genes Expression and Intramuscular Fat Content of Rose Cockscomb Chicken

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