TGFβ3基因多态位点及其与猪脊椎数性状的关联分析

doi:10.16431/j.cnki.1671-7236.2018.03.023

Abstract

Abstract:

This experiment was aimed to investigate single nucleotide polymorphism (SNP) within transforming growth factor beta 3 (TGFβ3) gene and its associations with the vertebral number of porcine(F2 generation resource population of Large White pig×Min pig).We screened the SNP within exons region of TGFβ3 gene in F0 generation individuals using PCR method and verified them in F2 generation,and analyzed its associations with vertebral number of porcine.The result showed that one SNP was found in F0 generation individuals (SSC7:105179474 G > A),which was devised into two genotypes:GG and GA.Association analysis between genotypes and vertebral number of porcine showed that SSC7:105179474 G > A was extremely significantly associated with thoracolumbar number and rib number (P < 0.01),and there was no significant association with the lumber number (P > 0.05).The χ² test demonstrated that this variation was in Hardy-Weinberg equilibrium (P > 0.05).The results demonstrated that TGFβ3 gene was primarily inferred to be a potential major gene or linked to the major gene affecting the vertebral traits of porcine,and SSC7:105179474 G > A might be a candidate molecular genetic markers to improve the vertebral number of porcine.

Key words: porcine; TGFβ3 gene; SNP; vertebral number

CLC Number:

S828

YUE Jingwei, GUO Hongzhou, ZHOU Weiwei, LIU Xin, WANG Ligang, GAO Hongmei, HOU Xinhua, ZHANG Yuebo, YAN Hua, WEI Xia, ZHANG Longchao, WANG Lixian. Polymorphism Sites of TGFβ3 Gene and Its Association Analysis with Vertebral Number of Porcine[J]. , 2018, 45(3): 738-744.

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Polymorphism Sites of TGFβ3 Gene and Its Association Analysis with Vertebral Number of Porcine

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