猪ROCK1基因克隆、序列分析及时空表达研究

doi:10.16431/j.cnki.1671-7236.2018.03.002

Abstract

Abstract:

To further obtain the information about the sequence structure,biology function and spatio-temporal expression of Rho-associated,coiled-coil containing protein kinase 1(ROCK1) gene for meat traits,ROCK1 gene was cloned through PCR and SMARTer RACE PCR,the sequence was analyzed by bioinformatics method,and the mRNA expression was detected by RT-PCR and quantitative Real-time PCR.The results showed that ROCK1 gene had two transcripts,both containd 4 065 bp open reading frame (ORF),encoding 1 354 amino acids,and the high sequence homology of ROCK1 gene among human,mice and rats were 95%,91% and 91%,while the corresponding amino acid sequence homology were 98%,96% and 94%,respectively.ROCK1 protein was highly conserved in different species.ROCK1 gene expressed widely in different tissues,and the expression level changed in varied development stages.The ROCK1 gene expression of embryonic longisimus dorsi in Meishan pigs was extremely significantly higher than that of Yorkshire pigs (P < 0.01),while there were no significant differences in other developmental stages after birth (P > 0.05).The results would provide a basis for further study of the biological function and mechanism of ROCK1 gene on porcine skeletal development.

Key words: porcine; ROCK1 gene; cloning; expression

CLC Number:

FENG Xiaoting, ZHU Jiping, ZHANG Ruirui, LI Yi. Cloning, Sequence Analysis and Expression Patterns of Porcine ROCK1 Gene[J]. , 2018, 45(3): 571-580.

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Cloning, Sequence Analysis and Expression Patterns of Porcine ROCK1 Gene

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