芦花鸡IFITM3基因克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2018.02.006

Abstract

Abstract:

This study was aimed to clone the interferon-induced transmembrane protein 3(IFITM3) gene of Luhua chicken and analyze its genetic structure with bioinformatics. One pair of special primers were designed according to predicted sequence of chicken IFITM3 gene in GenBank (accession No.:NM_001350061.1). The IFITM3 gene of Luhua chicken was amplified by RT-PCR, the physicochemical property and secondary structure of IFITM3 gene were systemically analyzed by bioinformatics. The results showed that the IFITM3 gene fragment included a 414 bp whole length CDS (coding 137 bp amino acids), and the gene sequence homology was above 99.9% comparison with the sequence published in GenBank (accession No.:NM_001350061.1). The molecular weight, isoelectric point, instability index and fat coefficient of IFITM3 were 14.95 ku,6.89,33.98 and 103.14, respectively. The prediction result of IFITM3 gene structure showed that IFITM3 had no signal peptide but had three obvious hydrophobic domains, and the average hydrophobic index was 0.300. The two transmembrane domains might locate in the 46 to 68 and 101 to 123 amino acids across the online TMHMM 2.0 software. The prediction of secondary structure revealed that some α-helixes and β-sheets exist in IFITM3 protein while random coil was major pattern. The results suggested that the whole CDS sequence of Luhua chicken IFITM3 gene was successfully cloned, which provided foundation for further studying on the function and antiviral molecular mechanisms of Luhua chicken IFITM3 protein.

Key words: IFITM3 gene; clone; sequence analysis; bioinformatics analysis

CLC Number:

JIANG Lili, XU Teng, ZHOU Wanrong, FAN Zhaobin. Cloning and Bioinformatics Analysis of Interferon-induced Transmembrane Protein 3(IFITM3) Gene in Luhua Chicken[J]. , 2018, 45(2): 330-337.

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Cloning and Bioinformatics Analysis of Interferon-induced Transmembrane Protein 3(IFITM3) Gene in Luhua Chicken

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