驴Zfy基因cDNA克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2018.01.005

Abstract

Abstract:

This study was aimed to clone the cDNA sequence of Equus asinus Zfy gene and analyzed its genetic structure with bioinformatics. Specific primers were designed according to the mRNA sequence (accession No.:NM_177491.1) of Bos taurus Zfy gene published in GenBank, and Zfy gene were amplified and cloned by RT-PCR. The nucleotide sequence and protein structure of CDS region were analyzed through bioinformatic method. The results showed that the length of CDS region of Equus asinus Zfy gene was 2 325 bp, encoding 774 amino acids. Compared with Bos taurus, Ovis aries, Homo sapiens, Felis catus, Canis lupus, Cervus elaphus, Pan troglodytes and Pan tholops hodgosonii, the homology of the CDS region of Equus asinus Zfy gene were 92.9%, 92.6%, 91.8%, 93.8%, 92.5%, 92.3%, 91.6% and 92.6%, respectively. The phylogenetic tree, constructed from the CDS region of Equus asinus and eight other mammalian Zfy gene cDNA, displays that Equus asinus and Ovis aries, Pantholops hodgosonii, Bos taurus, Cervus elaphus had a closer genetic relationship, however the relationship was far away among Equus asinus, Felis catus and Canis lupus, and the relationship with Homo sapiens and Pan troglodytes was the farthest. In this study, we cloned the CDS region of Equus asinus Zfy gene, which laid the foundation for the further study of the function of Zfy gene.

Key words: Equus asinus; Zfy gene; cloning; bioinformatics

CLC Number:

YING Ruiwen, ZHANG Yongsheng, XIA Huan, WANG Xiangzu, LI Chaocheng, WANG Xuhai, XI Jifeng, JIA Bin. cDNA Cloning and Bioinformatics Analysis of Zfy Gene in Equus asinus[J]. , 2018, 45(1): 39-46.

References

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cDNA Cloning and Bioinformatics Analysis of Zfy Gene in Equus asinus

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