斑点叉尾鮰套肠病病原嗜水气单胞菌的分离与鉴定

doi:10.16431/j.cnki.1671-7236.2017.10.032

Abstract

Abstract:

To ensure the cause of suspected bacteria disease for channel catfish, the bacteria from clinical cases were isolated and cultured, then the biochemical and molecular biology assay, and animal regression test were used to identify the species of the isolated bacteria, bacteria medicine sensitive test was used to detect the bacterial drug resistance. The results showed that the bacteria were gram negative bacilli. The colony morphology and biochemical characteristics of the isolated bacteria were same as Aeromonas hydrophila. The sequencing test of the 16S rDNA also confirmed the isolated bacteria were Aeromonas hydrophila. The isolated bacteria could infect the healthy channel catfish and cause the same symptoms as the clinical case. The isolated bacteria were sensitive to floroquinolones and resistant to penicillin and cephalosporin drugs. The results showed that the pathogen of case catfish bowel disease was Aeromonas hydrophila, and the floroquinolones could use to prevent and treat the disease.

Key words: channel catfish; Aeromonas hydrophila; isolation and identification

CLC Number:

S943

WU Xuan, YUAN Hai-wen, XIE Xiao-dong, LI Zhu-xuan, GOU Chang-yong, LUO Wei, CHENG Zhen-tao, LI Pan. Isolation and Identification of Aeromonas hydrophila from the Case of Channel Catfish Bowel Disease[J]. , 2017, 44(10): 3057-3062.

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Isolation and Identification of Aeromonas hydrophila from the Case of Channel Catfish Bowel Disease

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