PPARγ对猪血管内皮细胞增殖、迁移及小管形成的影响

doi:10.16431/j.cnki.1671-7236.2017.09.037

Abstract

Abstract:

In order to investigate the effect of peroxisome proliferator-activated receptors γ (PPARγ) on proliferation, migration and tube formation of swine vein endothelial cells, and explore its role in swine placental angiogenesis, PPARγ agonist group (5,10,15 and 20 μmol/L rosiglitazone), inhibitor group (5, 10, 15 and 20 μmol/L T0070907) and control group were set in this study. Through iCelligence cell function analyzer, scratch test, Matrigel matrix three dimensional cell culture, a model of angiogenesis of swine vein endothelial cells in vitro was established for simulating the environment of pig angiogenesis in vivo and detecting the proliferation, migration, tube formation ability of cells. A pair of specific primer was designed by Primer Premier 5.0 software according to PPARγ sequence from NCBI. SYBR GreenⅠ Real-time PCR were performed to detect relative quantity of PPARγ gene mRNA and verify the effect of PPARγ on cells in vitro. The results showed that 5 and 10 μmol/L rosiglitazone could promote proliferation, migration and tube formation of swine vein endothelial cells, T0070907 had inhibition on cells and negatively correlated with concentration (5 to 15 μmol/L),both of rosiglitazone and T0070907 at high concentration (20 μmol/L) interfere in cells activity because of toxic effect. In addition, rosiglitazone (5 to 20 μmol/L) and T0070907 (5 to 20 μmol/L) increased and reduced relative quantity of PPARγ gene mRNA,respectively, and its trends of concentration were similar with the former three experiment. The results indicated that the activation of PPARγ promoted proliferation, migration and tube formation of swine vein endothelial cells, and it might play a positive role in swine placental angiogenesis.

Key words: PPARγ; swine; vein endothelial cells

CLC Number:

S814.1

PENG Xuan, XUE Li-qun, ZHANG Ju-zuo, YUAN An-wen. Effect of PPARγ on Proliferation, Migration and Tube Formation of Swine Vein Endothelial Cells[J]. , 2017, 44(9): 2809-2815.

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Effect of PPARγ on Proliferation, Migration and Tube Formation of Swine Vein Endothelial Cells

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