6株猪流行性腹泻病毒贵州株S基因序列分析

doi:10.16431/j.cnki.1671-7236.2017.06.007

Abstract

Abstract:

In order to understand the characteristics of S gene of porcine epidemic diarrhea virus (PEDV) in Guizhou province, and grasp the genetic variation of PEDV, according to 3 pairs of specific primers designed in the test, S gene of 6 strains of PEDV from Guizhou were amplified by RT-PCR, cloned and sequenced. Nucleotide sequence and phylogenetic tree of 6 strains of PEDV from Guizhou and reference strains were analyzed by biological information software. The result showed that the length of S gene genome of 6 strains of PEDV from Guizhou was 4 161 bp, encoding 1 387 amino acids, and the nucleotide homologies of domestic and foreign PEDV reference were from 93.3% to 98.8%, the amino acid identity were from 91.6% to 98.9%. Phylogenetic tree analysis results showed that the 6 strains were close to the American strain, Vietnam strain and the Shandong strain.They were far from the CV777, LZC, Brl and 83P-5.The experiment showed that the S gene of PEDV in Guizhou had a certain degree of amino acid change in recent year with a trend of variation. The study provided a theoretical basis for the prevention and control of PEDV in Guizhou province.

Key words: porcine epidemic diarrhea; S gene; bioinformatics analysis

CLC Number:

S852.65

MAO Li-hong, ZHANG Shuang-xiang, ZHOU Bi-jun, WANG Wei, HU Xing-yi, CHENG Zheng-tao, WEN Ming, WANG Kai-gong. Sequence Analysis of S Gene of 6 Strains of Porcine Epidemic Diarrhea Virus from Guizhou[J]. , 2017, 44(6): 1621-1629.

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Sequence Analysis of S Gene of 6 Strains of Porcine Epidemic Diarrhea Virus from Guizhou

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