猪嗜淋巴细胞疱疹病毒2型GD27株gB基因生物信息学分析

doi:10.16431/j.cnki.1671-7236.2017.01.026

Abstract

Abstract:

The glycoproteins B (gB) gene of porcine lymphotropic herpesviruses 2 (PLHV-2)was amplified by PCR and sequenced, then bioinformatics analysis were conducted. The gB protein was composed of 876 amino acids, the molecular formula was C₄₅₀₀H₆₉₇₅N₁₁₉₉O₁₃₅₁S₄₀, the molecular mass was 100.77 ku, the value of theoretical isoelectric point was 6.45, and the instability index was 38.58. The prediction of secondary structure revealed that some alpha helixes and beta sheets exist in protein while random coil was major pattern. It had a signal peptide in the position 1-39 amino acids and a transmembrane helice in the position 761-780 amino acids. It contained several prosites and several intrinsically disordered proteins. Tertiary structure model of gB protein showed a fusion loop. Multiple antigenic epitope located in gB through comprehensive analysis prediction method. It was suggested that gB gene could be as a candidate antigen for vaccination of PLHV-2.

Key words: porcine lymphotropic herpesviruses; gB gene; bioinformatics analysis

CLC Number:

S852.65⁺9.1

ZHU Shi-kang, TONG Tie-zhu, LIU Xing, LI Chun-ping, CHEN Yan-zhong, LUO Zhuo-jun, LIN Zhi-xiong, LIU Zhong-yong, ZHOU Yu. Bioinformatics Analysis of gB Gene of Porcine Lymphotropic Herpesviruses Strain GD27[J]. , 2017, 44(1): 186-193.

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Bioinformatics Analysis of gB Gene of Porcine Lymphotropic Herpesviruses Strain GD27

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