PGI2对缺少内源性PGs的绵羊早期胚胎体外发育的影响

doi:10.16431/j.cnki.1671-7236.2016.11.031

Abstract

Abstract:

This study was conducted to investigate the effect of PGI₂ on early embryo development of sheep in vitro lacking of endogenous prostaglandins（PGs).The specific inhibitor NS398 and SC560（specifically inhibiting the activity of COX-1 and/or COX-2）were added into the culture medium respectively to study the effect of COX-1 and COX-2 on embryonic development of sheep.Different concentrations of Iioprost were added respectively into the culture medium to investigate the effect of PGI₂ on early embryo development of sheep in vitro.The results showed that there were no significant differences on the cleavage and blastocyst rates between the group only with NS398 and that with both NS398 and SC560（P>0.05).There were significant differences between the group only with NS398 and control group（P<0.05).After adding NS398,different concentrations of exogenous Iloprost could replace the role of embryonic-derived PGI₂,which substantially eliminated the adverse effects of COX-2 inhibitor on early embryo development in vitro.The appropriate concentration of Iloprost was 1×10^-6 mol/L.H33342 staining showed no significant difference（P>0.05).The results demonstrated that embryonic endogenous COX-2 played a major role in regulating synthesis of PGI₂ in early embryo of sheep.The exogenous Iloprost was able to compensate for the loss of endogenous PGI₂ in embryos and remove the inhibition of NS398 on COX-2,ultimately promoting the development of early sheep embryos in vitro.

Key words: PGI₂; ovine; early embryo; in vitro development

CLC Number:

S814.8

LI He-juan, QIN Guo-song, QI Xiao-long, SHENG Xi-hui, LIU Yun-hai, GUO Yong, NI He-min. Effect of PGI₂ on Potential Development of in vitro Produced Ovine Embryos Which Lacking with Their Endocrinal PGs[J]. , 2016, 43(11): 3019-3023.

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Effect of PGI₂ on Potential Development of in vitro Produced Ovine Embryos Which Lacking with Their Endocrinal PGs

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Effect of PGI2 on Potential Development of in vitro Produced Ovine Embryos Which Lacking with Their Endocrinal PGs

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Effect of PGI₂ on Potential Development of in vitro Produced Ovine Embryos Which Lacking with Their Endocrinal PGs