PCV2通过NF-κB/NLRP3信号通路调控体外培养PAMs分泌IL-1β

doi:10.16431/j.cnki.1671-7236.2016.09.022

Abstract

Abstract:

This study investigated the molecular mechanism of interleukin-1β (IL-1β) production in porcine alveolar macrophages (PAMs),which were infected by porcine circovirus 2 (PCV2).Two 6-week-old piglets were selected as experiment animals,which were free of PCV2 and porcine reproductive and respiratory syndrome virus (PRRSV) antibody and antigen.The alveolar macrophages were isolated aseptically and cultured in vitro as this study object.IL-1β in the supernatants from PCV2-infected PAMs was assayed by ELISA.The expressions of NLRP3 and ASC in the PCV2-infected PAMs were detected by Real-time quantitative PCR.The regulatory function of NLRP3 and NF-κB in IL-1β secretion by the PCV2-infected PAMs was analyzed by small interfering RNA (siRNA) and NF-κB inhibitor BAY11-7082,respectively.The results showed that the expressions of IL-1β,NLRP3 (except for 1 h) and ASC (except for 1 and 3 h) in cell culture supernatants or PCV2-infected PAMs were significantly increased(P<0.05;P<0.01).siRNA could lead to 58.3% of NLRP3 gene silencing.IL-1β secretion in the PCV2-infected PAMs,which were companied by NLRP3 gene silencing,was significantly decreased (P<0.05).The IL-1β level in the PCV2-infected PAMs,which were treated with inhibitor BAY11-7082,was also significantly decreased.These results suggested that PCV2 regulated PAMs secreting IL-1β through the NF-κB/NLRP3 signal pathway in vitro.

Key words: PCV2; PAMs; IL-1β; NF-κB; NLRP3

CLC Number:

Q785

LI Hai-hua, ZHANG Lei, YANG Chun-lei, ZHAO Xiang-hua, QIAO Jia-yun, WANG Wen-jie. PCV2 Regulates PAMs Secreting IL-1β through the NF-κB/NLRP3 Signalling Pathway in vitro[J]. , 2016, 43(9): 2366-2372.

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PCV2 Regulates PAMs Secreting IL-1β through the NF-κB/NLRP3 Signalling Pathway in vitro

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