一株藏绵羊源短小芽孢杆菌的分离鉴定及生物学特性研究

doi:10.16431/j.cnki.1671-7236.2016.06.032

Abstract

Abstract: In order to select probiotic strains with probiotic potential isolated from Tibetan sheep,fresh excreta of the healthy female Tibetan sheep was processed with 90 ℃ water-curing treatment for 5 min,and Bacillus with resistance were screened.The morphological,physiological and biochemical characteristics and 16S rDNA sequence of the Bacillus were comprehensively analyzed and identified.Biochemical characteristics were studied by using antimicrobial test,acid resisting tests and bile salt resisting tests,hydrolytic enzyme test and other methods.The results showed that the Bacillus SWUN-G43 isolated from fresh excreta of Tibetan sheep had a significant antibacterial effect on E.coli and Salmonella.Through analysis and identification,the Bacillus SWUN-G43 was Bacillus pumilus.Its survival rate was 50.43% under the environment of pH 2.0 and 44.35% under the environment of 0.3% bile salt.It could produce protease and cellulase and was sensitive to 12 kinds of commonly used antibiotics.By studying,the Bacillus pumilus(SWUN-G43) had a certain degree of resistance and potential probiotic ability.Besides,the strain didn't contain R-plasmid and would not cause horizontal transmission of antibiotic resistance in the intestinal microecosystem.It also had the potential to be developed as a micro ecological additive.

Key words: Tibetan sheep; Bacillus pumilus; isolation; identification; characteristics

CLC Number:

S852.6

GUO Zheng-hong, ZHOU Biao, YAN Heng-xiu. Study on the Isolation,Identification and Biological Characteristics of a Strain of Bacillus pumilus Isolated from Tibetan Sheep[J]. , 2016, 43(6): 1610-1617.

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Study on the Isolation,Identification and Biological Characteristics of a Strain of Bacillus pumilus Isolated from Tibetan Sheep

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