硒化党参多糖对免疫抑制小鼠免疫功能的影响

doi:10.16431/j.cnki.1671-7236.2016.06.022

Abstract

Abstract: The study was aimed to observe the effects of Codonopsis pilosula polysaccharides(CPPS) and selenium-Codonopsis pilosula polysaccharides₅(sCPPS₅) on the immune functions of immunosuppressive mice induced by cydophosphamide (CY).96 mice were randomly divided into 8 groups:Normal control group,CY model group,CY+CPPS (low,middle and high dose groups) and CY+sCPPS₅ (low,middle and high dose groups).Mice in normal control group and CY model group were lavaged with normal saline,the mice in other groups were lavaged with CPPS and sCPPS₅ by 0.2,0.4 and 0.6 mg/g,respectively.Administration was carried out once a day for 10 successive days.On the 11th day,mice were manufactured immunosuppressed by a three-day intraperitoneal injection of 0.04 mg/g CY,except for the normal control group.After administrated with drugs for 24 h,the immunological indexes of the mice were detected.The results showed that spleen and thymus indexes of CPPS and sCPPS₅ groups were significantly higher than that of CY group (P < 0.05).The A_{570 nm} when stimulated with ConA and LPS,serum IgG and IgM contents of CPPS and sCPPS₅ groups were significantly higher than that of CY group (P < 0.05) and sCPPS₅ possessed better efficacy than CPPS.In conclusion,CPPS and sCPPS₅ could strengthen immune activity of the immunosuppressive mice,but sCPPS₅ was better than CPPS and selenylation modification could improve the immune activity of polysaccharide.

Key words: sCPPS₅; CPPS; immune function; lymphocyte; immune globulin

CLC Number:

S852.2

LIN Dan-dan, QIN Tao, REN Zhe, MA Yu-fang, LI Jian, HUANG Yi-fan. Effects of Selenium-Codonopsis pilosula Polysaccharide on Immune Function of Immunosuppressed Mice[J]. , 2016, 43(6): 1544-1549.

References

[1] 中华人民共和国药典委员会.中华人民共和国药典[M].北京:化学工业出版社,2000.
[2] 焦红军.党参的药理作用及其临床应用[J].临床医学,2005,25(4):92.
[3] 孙德君,张立恒,姜宇,等.中药多糖免疫增强作用及机理的研究进展[J].中国畜牧兽医,2011,38(8):240-243.
[4] 杨丰榕,李雨潮,郭小龙,等.党参多糖研究进展[J].内蒙古中医药,2014,36:106-107.
[5] 张兆林,兰中芬,王凤连,等.党参多糖成分及其免疫药理作用研究[J].兰州医学院学报,1988,3:14-15.
[6] 李达,李在林.党参多糖作为免疫佐剂的研究综述[J].中国医药指南,2013,28:56-57.
[7] 晏永新,张丽,贾海芳,等.党参多糖口服液对小鼠免疫功能的影响[J].中国兽药杂志,2013,47(3):18-20.
[8] 崔乔,尚德静,邹霞.硒多糖的研究进展[J].中国生化药物杂志,2003,24(3):155-157.
[9] Wang J L,Zhao B T,Wang X F,et al.Synthesis of selenium-containing polysaccharides and evaluation of antioxidant activity in vitro[J].International Journal of Biological Macromolecules,2012,51(5):987-991.
[10] 张晓君,祝晨,胡黎,等.党参多糖对小鼠免疫和造血功能的影响[J].中药新药与临床药理,2003,14(3):174-176.
[11] Sun Y X,Liu J C.Structural characterization of a water-soluble polysaccharide from the roots of Codonopsis pilosula and its immunity activity[J].International Journal of Biological Macromolecules,2008,43(3):279-282.
[12] 郭晓农,戚欢阳,王兵,等.党参多糖抗氧化作用及其对果蝇寿命的影响[J].食品科学,2013,34(15):285-288.
[13] Liu C,Chen J,Li E T,et al.The comparison of antioxidative and hepatoprotective activities of Codonopsis pilosula polysaccharide (CP) and sulfated CP[J].International Immunopharmacology,2015,24(2):299-305.
[14] 马方励,沈雪梅,时军.党参多糖对实验动物胃肠道功能的影响[J].安徽医药,2014,18(9):1626-1629.
[15] 冯浩丽,高建平.党参多糖体内抗肿瘤活性研究及急性毒性实验[J].山西中医,2012,28(8):49-50.
[16] Xin T,Zhang F B,Jiang Q Y,et al.The inhibitory effect of a polysaccharide from Codonopsis pilosula on tumor growth and metastasis in vitro[J].International Journal of Biological Macromolecules,2012,51(5):788-793.
[17] 弋清,罗霞,陈东辉,等.不同剂量环磷酰胺诱导正常小鼠免疫抑制的对比研究[J].免疫学杂志,2005,21(3):122-124.
[18] 崔治中,崔保安.兽医免疫学[M].北京:中国农业大学出版社,2010.
[19] 贾林,陆金健,周文雅,等.桔梗多糖对环磷酰胺诱导的免疫抑制小鼠的免疫调节[J].食品与机械,2012,28(3):112-114.
[20] 王书全.酵母多糖对免疫抑制小鼠的免疫调节作用[J].现代畜牧兽医,2015,5:1-6.
[21] 赵现敏,崔保安,张红英,等.4种中药多糖对猪脾淋巴细胞增殖的影响[J].河北农业大学学报,2007,30(3):82-85.
[22] 王洪鸽,汤承,王航,等.白花蛇舌草多糖对小鼠免疫功能和生长发育的影响[J].中国畜牧兽医,2013,40(10):140-143.
[23] 佟春玉,孙越春,秦学功,等.天门冬多糖对环磷酰胺诱导的免疫抑制小鼠的免疫调节作用[J].天然产物研究与开发,2013,8:1112-1114.
[24] 凌云,单晶,张雅琳,等.中药蒲公英对小鼠脾淋巴细胞增殖的影响[J].解放军药学学报,2005,21(1):73-74.
[25] Harry W S,Lisa C.Structure and function of immunoglobulins[J].The Journal of Allergy and Clinical Immunology,2010,125(2):41-52.
[26] 许爱华,任莉,郑媛媛,等.银杏外种皮多糖对环磷酰胺诱导的免疫抑制小鼠免疫反应的调节作用[J].中国药理学与毒理学杂志,2008,22(1):69-72.
[27] 许丹妮.金线莲多糖对小鼠免疫调节作用的研究[D].神州:福建农林大学,2011.

Effects of Selenium-Codonopsis pilosula Polysaccharide on Immune Function of Immunosuppressed Mice

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LU Hui, CAI Gaofeng, WU Caihong, QIU Shulei, YUAN Chen, CHEN Xiaolan, LIU Yun, QIN Kaili. Effect of Agrocybe aegerita Polysaccharid on Immunomodulatory of Splenic Lymphocytes in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1828-1835.
[2]	HU Honglian, LI Chao, CHEN Liqing, SONG Liwen, ZHAO Meng, ZHANG Jianxia, WANG Dian, GAO Min. Effects of Dietary β-carotene Supplementation on Lactation Performance, Blood Routine,Serum Biochemical and Immune Indexes of Dairy Cows during the Lactating Peak Period [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1375-1384.
[3]	LIU Zhen, ZHOU Xueqin, XU Fei, LI Zhefeng, SUN Hanxue, LI Guoqin, TAO Zhengrong, GUAN Mei, ZHENG Qiang, DIAO Xinping, LU Lizhi. Effects of Coated Sodium Butyrate on Growing Performance,Slaughter Performance, Immune Organ Index and Digestive Enzyme Activity of Breeding Pigeons and Squabs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 998-1006.
[4]	LIU Zhenni, LEI Xiaowen, LI Jianjun, ZENG Qingyuan, TAN Donghai, ZHONG Yunping, ZHANG Qiang, XIE Hualiang. Effects of Fermented Navel Orange Pomace on Intestinal Morphology,Intestinal Mucosal Immune Function and Microbial Diversity of Muscovy Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 573-585.
[5]	ZHAO Luyuan, ZOU Chenhao, CAO Jiu'ai, GUO Yanshan, LIU Lijun, ZHAN Xiu'an, LIN Gang, LIU Bing, YU Dongyou. Effects of Low Level of Compound Organic Trace Minerals on Serum Biochemistry,Immune Function and Antioxidant Capacity of Meat Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 86-96.
[6]	WANG Xuefang, GUI Rong, CHEN Gongyi, WANG Wei, ZHANG Hongying, WANG Xuebing, LI Xiao. Clinical Study of Lianhuachaiqin Soluble Powder on Chicken Syndrome of Wind-heat Invading Lung [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3610-3621.
[7]	LI Hongying, HE Qin, HUANG Enfu, LIANG Qi. Effects of Plant Essential Oils on Immune Function, Antioxidant Capacity and Gut Microbiota of Shan Partridge Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2586-2592.
[8]	YUE Fuyuan, ZHU Lianqin, CHEN Fu, ZHANG Beibei, ZHU Fenghua. Effects of Organic Organically Modified Montmorillonite Added to Mildew Feed on Growth Performance and Immune Function in Chicks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2137-2144.
[9]	BAI Peitian, KONG Jiamei, PEI Ting, CHENG Zhixue, REN Yuhong. Effects of Compound Microecological Preparation on Growth Performance, Immune Function and Caecal Flora Structure of Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 942-952.
[10]	LI Shinian, LIU Wanning, CHEN Yaping, WANG Jintao. Design of Multi Epitope Vaccine Against Swine Acute Diarrhea Syndrome Coronavirus S, M and E Proteins Based on Immunoinformatics Methods [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1057-1066.
[11]	YU Xiaojun, LI Dandan, LI Xuewu, WANG Qun, WANG Guang, WANG Lihua. Effects of Antibacterial Peptide on the Immune Function and Intestinal Tissue Morphology of Growing Female Minks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 443-453.
[12]	GU Dongdi, DENG Zaishuang, REN Man, SUN Yuhao, GU Youfang, JIN Guangming, JIN Erhui. Effects of Cordyceps Powder Combined with Yeast Selenium on Laying Performance,Egg Quality,Antioxidant and Immune Function in Middle Egg-laying Peak of Laying Hen [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2404-2414.
[13]	WANG Yibing, KUANG Zhixiang, YE Jinling, FAN Qiuli, ZHANG Sheng, JIANG Shouqun. Effects of Nano Copper,Spores of Ganoderma lucidum and Soybean Isoflavone on Performance of Growth,Immune and Antioxidation of Qingyuan Partridge Chickens from 1 to 30 Days [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2415-2423.
[14]	ZHAO Yi, WEN Luting, HUANG Yin, CHEN Zhong, LIU Qian, LIN Yong, HU Tingjun. Effects of Sophora subprosrate Polysaccharide on Growth Performance and Immune Function of Oreochromis niloticus [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3635-3643.
[15]	SONG Yukun, HE Jun. Research Progress on Structure,Function and Its Key Immune Genes of Spleen in Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1709-1718.