›› 2016, Vol. 43 ›› Issue (4): 1024-1031.doi: 10.16431/j.cnki.1671-7236.2016.04.026

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Microsatellite Analysis of Six Chinese Indigenous Sheep Breeds

QIU Xiao-yu, HUANG Yong-fu, ZHAO Yong-ju, NA Ri-su   

  1. College of Animal Science and Technology, Southwest University, Chongqing 400716, China
  • Received:2015-10-13 Online:2016-04-20 Published:2016-04-27

Abstract: This article described the relationship among six Chinese indigenous sheep breeds.There were six microsatellite loci which used to analyze the genetic diversity.Based on the allele frequency of six sheep variations, we calculated the mean heterozygosity (Ho), which were from 0.5446 to 0.7140;The polymorphism information content (PIC) in six sheep breeds were from 0.5211 to 0.7732.Genetic variations within breed were analyzed in the study, and we clustered the UPGMA tree according to the DA genetic distance.The results showed that six breeds had relatively abundant genetic diversity, and MXB had the highest genetic variation, while SW had the lowest genetic variation.The inbreeding coefficient of SW was higher than other breeds, which revealed a higher inbreeding degree in this breed.Phylogenetic tree was constructed using UPGMA, which showed that LZD and MXB were classified as a class, HZK and GF were classified as a class, SIH and SW were classified as a class.The results revealed the genetic structure of breeds correlated with geographical distribution and history of domestication.

Key words: microsatellite; genetic diversity; sheep

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