肥肝鹅恢复期血液生化指标、肝脏常规营养成分及脂代谢相关基因表达水平的变化研究

doi:10.16431/j.cnki.1671-7236.2016.04.017

Abstract

Abstract: This experiment aimed to determine the changes of body weight, liver weight, abdominal fat weight, hepatic routine nutritional composition, blood biochemistry indexes, as well as mRNA expression of lipid metabolism-associated genes during the recovery of Landes geese with fatty liver, which provided the basis for the further elucidation of the recovery or protection mechanism of goose fat liver.18 Landes geese were divided into three groups (6 gees in each group).Group 1 was control group, in which the geese were fed with boiled maize;Group 2 was the overfeeding group without recovery, in which the geese were overfed with a maize-based diet for 19 days;And group 3 was the overfeeding group with 20-day recovery, in which the geese were overfed with the maize-based diet for 19 days followed by feeding boiled maize for 20-day recovery.The results showed that compared with group 2, the body weight and liver weight of the geese in group 3 decreased extremely significantly (P<0.01), and the abdominal fat weights undecreased significantly (P>0.05);Moreover, the percentages of nutrients including water, ash, CP of livers in group 3 increased significantly (P<0.05), while the percentage of EE decreased significantly (P<0.05), but there were no significant difference for these variables between groups 1 and 3 (P>0.05) except water.In regard to blood biochemistry indexes, the contents of blood ALT, AST, TG and HDL-C in group 3 decreased significantly (P<0.05), but there were no significant difference from those of group 1 (P>0.05).The mRNA expression levels of FADS1, SCD1 and CYP2C45 in group 3 decreased significantly compared with group 1 (P<0.05), but there were no significant difference between groups 1 and 3 (P>0.05).In summary, this study demonstrated the recoverability of goose fatty liver at different layers, and thus laid a foundation for further investigation on the recovery or protective mechanism of goose fatty liver and found the solution to resolve the problems related to animal fatty liver.

Key words: Landes goose; overfeeding; fatty liver; biochemical indexes; recovery; lipid metabolism

CLC Number:

S852.2

XIA Li-li, WANG Qian-qian, YANG Biao, SUN Xiao-xian, ZHANG Yi-hui, LIU Long, GENG Tuo-yu, GONG Dao-qing. Study on Changes of Blood Chemistry Indexes,Hepatic Routine Nutritional Composition and Expression of Lipid Metabolism-associated Genes during the Recovery of Geese with Fatty Livers[J]. , 2016, 43(4): 967-972.

References

[1] Yasuhara M,Ohama T,Matsuki N,et al.Induction of fatty liver by fasting in suncus[J].J Lipid Res,1991,32(6):887-891.
[2] Davail S,Guy G,Andre J,et al.Metabolism in two breeds of geese with moderate or large overfeeding induced liver-steatosis[J].Comp Biochem Physiol A Mol Integr Physiol,2000,126(1):91-99.
[3] Mourot J,Guy G,Lagarrigue S,et al.Role of hepatic lipogenesis in the susceptibility to fatty liver in the goose (Anser anser)[J].Comp Biochem Physiol B Biochem Mol Biol,2000,126(1):81-87.
[4] Davail B,Pakdel F,Bujo H,et al.Evolution of oogenesis:The receptor for vitellogenin from the rainbow trout[J].J Lipid Res,1998,39(10):1929-1937.
[5] Fournier E,Peresson R,Guy G,et al.Relationships between storage and secretion of hepatic lipids in two breeds of geese with different susceptibility to liver steatosis[J].Poult Sci,1997,76(4):599-607.
[6] 王来娣,邵丹,张宜辉,等.朗德鹅填饲期肝重及肝脏营养成分的变化规律研究[J].中国家禽,2013,35(5):27-29.
[7] 朱丽慧,武艳军,关佳佳,等.填饲对朗德鹅血液指标、组织营养成分以及肝脏组织学的影响[J].中国家禽,2010,32(3):28-31.
[8] 张蕊,张宜辉,洪胜辉,等.填饲期朗德鹅血浆生化指标变化规律及其与产肝性能关系[J].扬州大学学报,2011,32(1):73-77.
[9] Geng T,Xia L,Li F,et al.The role of endoplasmic reticulum stress and insulin resistance in the occurrence of goose fatty liver[J].Biochem Biophys Res Commun,2015,465(1):83-87.
[10] 张蕊.鹅CYP2C45基因的结构功能及其与肥肝生产性能关系的分析[D].扬州:扬州大学,2014.
[11] 王惠影,刘毅,王翠,等.乳酸菌对朗德鹅填饲效果的影响[J].中国畜牧兽医,2015,42(5):1152-1157.
[12] Hermier D.Lipoprotein metabolism and fattening in poultry[J].J Nutr,1997,127(5):805S-808S.
[13] 王宝维,舒常平,葛文华,等.填饲期肥肝鹅脂肪沉积、血脂成分和脂类代谢酶的变化规律[J].中国农业科学,2014,47(8):1600-1610.
[14] 曹苹,何佳,邝辉,等.脂肪酶对大鼠非酒精性脂肪肝的防治作用[J].中国现代应用药学,2011,28(13):1301-1303.
[15] 蔡洁娜,胡志明,张犁,等.口服脂肪酶对高脂性模型大鼠脂代谢和脂肪肝的影响[J].广东医学,2014,35(7):989-992.
[16] 周联高,刘巧泉,王捍东,等.转基因稻谷日粮对肉仔鸡血清生化指标的影响[J].饲料工业,2009,32(11):17-19.
[17] 王健,朱振鹏,董飚,等.番鸭填饲期血浆生化指标变化及其与产肝性能的相关性分析[J].中国畜牧兽医,2013,40(12):104-107.
[18] 韦光海.肝硬化患者血清总胆固醇、胆碱酯酶及尿酸的变化及其意义[J].医学理论与实践,2008,21(10):1138-1139.
[19] 孙宜梅,康相涛,王艳红,等.非洲鸵鸟血液生化指标的测定[J].广州农业科学,2008,2:83-84.
[20] 魏建军,何瑞国,范文娜,等.茶多酚对朗德鹅产肝性能和血清脂类成分的影响[J].中国饲料,2007,2:35-38.
[21] 杜荣,秦健,王俊东.动物脂质代谢调控的关键及其研究进展[J],动物科学与动物医学,2002,19(10):16-19.

Study on Changes of Blood Chemistry Indexes,Hepatic Routine Nutritional Composition and Expression of Lipid Metabolism-associated Genes during the Recovery of Geese with Fatty Livers

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Shuaijie, TAN Zihan, LI Hanbo, SUN Xiangxue, AI Sihan, WANG Lei, ZHAO Di, HOU Yongqing. Effects of Quercetin on Intestinal Morphology, Antioxidant Function and Jejunal Lipid Metabolism Gene Expression in Piglets with Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3391-3399.
[2]	WEI Jia, BAI Qin, LUO Xiaolin, GUAN Jiuqiang, AN Tianwu, ZHAO Hongwen, TAN Wu, LI Huade, XIE Rongqing, SHA Quan, JIANG Mingfeng, ZHANG Xiangfei. Effects of Different Weaning Strategies on Growth, Serum Biochemical Indexes and Antioxidant Capacity of Yak Calves [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3400-3410.
[3]	WANG Manli, TAN Zihan, WANG Shuaijie, LI Qian, ZENG Yitong, WANG Lei, ZHAO Di, ZHANG Qian, HOU Yongqing. Effects of Quercetin on Growth Performance, Blood Biochemical Indexes and Intestinal Absorption and Barrier Function in Piglets Infected with PEDV [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3411-3418.
[4]	CHEN Lihong, ZHAO Mengdi, LIU Keyuan, NING Haoran, CUI Kai, LI Guangyu, SUN Weili. Study on Optimum Dietary Metabolizable Energy Level of Male Pheasants from 13 to 17 Weeks of Age [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2943-2952.
[5]	REN Jianming, JI Peng, ZHANG Xiaosong, WEI Yanming, ZHU Xiaopeng, ZHU Yueming. Effects of Jianpi Xiaodao Chinese Herbal Compound on Growth Performance, Rumen Fermentation Parameters and Serum Indexes of 6-month-old Simmental Crossbred Cattles [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2593-2600.
[6]	LIANG Yefei, HUANG Panling, LI Shuhui, HUANG Guodong, XIA Xing, LI Yuqing. Effect of Mogrosides on Lipid Metabolism in Obese Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2129-2136.
[7]	SUN Qingyun, FAN Xiang, XING Ya, ZHAO Minmeng, LIU Long, GENG Tuoyu, GONG Daoqing. Study on the Relationship Between OTUD7A Gene and Goose Fatty Liver Formation [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1295-1303.
[8]	YANG Juan, GUO Shuai, HAN Shichang, ZHANG Nian, ZHANG Feng, TAO Hu, XIONG Qi. Study on the Differences of Blood Indexes and Slaughter Performance Between 6-month-old Boer Goats and Macheng Black Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1332-1342.
[9]	LIU Xinyong, TAN Zhengzhun, LI Hui, HUANG Jian, LUO Hua, ZENG Linghu, CHENG Juanru, ZHONG Huapei, YANG Chunyan, WANG Xiaoli, QIN Guangsheng. Effects of Heat Stress on Blood Biochemical Indexes of Murrah and Nili-Ravi Buffaloes [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 521-528.
[10]	CUI Chengyu, MA Ben, ZHAO Jiahui, ZHAO Zijuan, CHEN Yu, CAO Zhongzan, LUAN Xinhong. Application Prospect of Adiponectin and Its Receptor Agonists in Avian Fatty Liver Disease [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 738-745.
[11]	WANG Jiaxin, HAN Yunsheng, CAI Hongying, WEN Zhiguo, XU Xin, ZHAO Zitao, MENG Kun, YANG Peilong. Effects of Bacillus licheniformis on Growth Performance, Serum Biochemical Indexes and Intestinal Morphology of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4593-4603.
[12]	ZHANG Yuanyuan, TIAN Zhichen, SUN Yujia, ZHANG Huimin, YANG Zhangping, WANG Cong, CAO Ke, MAO Yongjiang, LI Mingxun. Effects of Taurine Supplementation on Glycolipid Metabolism in C57BL/6J Mice with High-fat Diet [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4604-4613.
[13]	LI Li, ZHU Pan, GUO Xinglan, PAN Bingqiang, ZHOU Jingkai, CHEN Jun. Effect of Crude Polysaccharide from Melastoma dodecandrum Lour. on Lipid Metabolism of Obese Mice Induced by High-fat Diet [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 122-130.
[14]	LU Liye, NIU Fugui, WANG Zhonghua, LIN Xueyan, WANG Yun, HOU Qiuling. Receiver Operating Characteristic Curve Analysis of Biomarkers of Fatty Liver in Perinatal Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 361-367.
[15]	SU Lingling, TUOHUTI Ajide, ZHANG Guoting, PAREHATIJIANG Wufuer, XIE Lirong, WANG Qiong, ABULAITI Sulaiman, NUERNISA Molaniyazi, REN Juan, ZHANG Yuhua, XIAO Hailong, XIE Peng, XIAO Haixia. Effects of Whole Corn Silage and Supplementary Sodium Bicarbonate on Growth Performance,Digestibility,Serum Biochemical Indexes and Economic Benefits in Jiangyue Donkey [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2388-2396.