牛MARK2、CREB5基因的克隆和生物信息学分析

doi:10.16431/j.cnki.1671-7236.2016.02.004

Abstract

Abstract: This research aimed to clone and analyze the biological characteristics of MARK2 and CREB5 genes in cattle.In the GenBank database,MARK2 and CREB5 genes of human were chosen to blast and expressed sequence tags (ESTs) of cattle were got.Specific primers were designed and RT-PCR was used to clone the cDNA sequence from the scapula muscle of cattle with vacancy parts making up,and then MARK2 and CREB5 genes were successfully cloned.The results showed that MARK2 gene coding region was 2 076 bp,which encoded 691 amino acids.CREB5 gene coding region was 1 527 bp,which encoded 508 amino acids.The physical and chemical properties of protein prediction showed that MARK2 and CREB5 proteins were hydrophilic protein;The phosphorylation sites in threonine (Thr),serine (Ser) and tyrosine (Tyr) residues;The secondary structure of MARK2 and CREB5 proteins were maily composed of random coil and layed a foundation for the tertiary structure;Protein subcellular localization prediction showed that MARK2 and CREB5 proteins did not belong to the secretory protein;MARK2 protein contained a S_TKc domain and an UBA function structure,CREB5 protein contained a ZnF_C2H2 function structure,a BRLZ function structure and five low complexity regions and MARK2 and CREB5 proteins had no transmembrane structure.

Key words: clone; MARK2 gene; CREB5 gene; bioinformatics

CLC Number:

YUE Ying-wei, WANG Xin, DU Miao, MA Wen-zhi, GUO Hong. Cloning and Bioinformatics Analysis of MARK2 and CREB5 Genes in Cattle[J]. , 2016, 43(2): 311-318.

References

[1] 张海军,尹俊亮,陈宁.牛肉品质的营养调控研究进展[J].中国草食动物,2008,28(2):60-61.
[2] 刘晓蕾,侯琳,蒋正尧,等.fat-1转基因小鼠的构建与鉴定[J].青岛大学医学院学报,2007,43(1):13-14.
[3] 杨慧婷,王洪梅,方永志,等.fat-1转基因动物研究进展[J].生物技术通报,2012,12:9-10.
[4] Kusakabe M,Nishida E.The polarity-inducing kinase Par-1 controls Xenopus gastrulation in cooperation with 14-3-3 and a PKC[J].EMBO J,2004,23(21):4190-4193.
[5] 齐鲁,丁彦青.大肠癌患者生存相关基因的筛选与网络调控分析[J].中国科学生命科学,2014,44(5):481-487.
[6] Qi L,Ding Y.Screening and regulatory network analysis of survival-related genes of patients with colorectal cancer[J].Sci China Life Sci,2014,57(5):526-531.
[7] 王冬冬,朱延明,李勇,等.电子克隆技术及其在植物基因工程的应用[J].东北农业大学生命科学学报,2006,37(3):403-408.
[8] 万发春,张幸开,张丽萍,等.牛肉品质评定的主要指标[J].中国畜牧兽医,2004,31(12):17-19.
[9] 罗红师.影响牛肉品质的因素[D].贵阳:贵州大学,2013.
[10] 刘丽.黄牛及其改良牛产肉性能和肉品质量分析及中国牛肉等级标准的研究与制度[D].南京:南京农业大学,2000.
[11] Zhao H L,Liu L Z,Sui Y,et al.Fatty acids inhibit insulin-mediated glucose transport associated with actin remodeling in rat L6 muscle cells[J].Acta Diabetol,2010,43(4):331-339.
[12] Hurov J B,Huang M,White L S,et al.Loss of the Par-1b/MARK2 polarity kinase lead to increased metabolic rate,decreased adiposity,and insulin hypersensitivity in vivo[J].Proc Natl Acad Sci USA,2007,104(13):5680-5685.
[13] 余瑞元,王燕峰,徐长法.CREB研究进展[J].中国生物工程杂志,2013,23(1):215-221.
[14] 刘伍限,贾斌,石国庆.绵羊FGF5基因编码蛋白的生物信息学分析[J].石河子大学学报(自然科学版),2011,29(3):296-300.
[15] 吴泽月,陈岳辉.蛋白质亚细胞定位预测方法研究进展[J].山东师范大学学报(自然科学版),2012,27(4):33-37.
[16] 钟茂春,郑光明,赵建,等.鲮Myf5基因克隆及其分析[J].中国水产科学,2010,17(4):681-687.
[17] 骆继怀,独军政,常惠芸,等.转基因克隆动物的发展及其应用[J].动物医学进展,2010,31(7):103-106.
[18] 张松,黄波,夏学峰,等.蛋白质亚细胞定位的生物信息学研究[J].生物化学与生物物理进展,2007,34(6):573-579.

Cloning and Bioinformatics Analysis of MARK2 and CREB5 Genes in Cattle

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