转基因猪中抗生素标记基因neo漂移风险评估

doi:10.16431/j.cnki.1671-7236.2015.10.031

Abstract

Abstract: This study was designed to study the possibility of gene drift of the antibiotic marker gene neo in transgenic pigs.First,dominance and recessiveness of neo gene in 6 piglets of F1 were identified using Southern blotting.It was found that 3 piglets were positive transgenic piglets and the other 3 piglets were non-transgenic piglets.The neo gene in blood and digestive tract tissue of experimental piglets were detected using PCR.The result showed that no gene drift was showed in blood and digestive tract tissues of experimental piglets.Then PCR technology was used to detect neo gene in intestinal bacteria of experimental piglets.The result showed that neo gene did not exist in the genome of intestinal bacteria.This study suggested that no gene drift was found in blood,intestinal bacteria and digestive tract tissues of piglets.

Key words: transgenic piglets; neo gene; gene drift

CLC Number:

WANG Qing-qing, GAO Peng-fei, LI He-gang, MA De-zun, CAI Chun-bo, QIAN Li-li, JIANG Sheng-wang, CUI Wen-tao. Risk Assessment of Antibiotic Marker Gene neo Drifts in Transgenic Pigs[J]. , 2015, 42(10): 2720-2725.

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Risk Assessment of Antibiotic Marker Gene neo Drifts in Transgenic Pigs

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