新生儿卵巢同源盒基因在猪卵巢中的表达研究

doi:10.16431/j.cnki.1671-7236.2015.10.028

Abstract

Abstract: Newborn ovary homeobox gene (NOBOX) is one of maternal genes,which is important in early oogenesis.In order to know the expression pattern of NOBOX gene in porcine tissues,Real-time fluorescence quantitative PCR was used to detected the expression of NOBOX gene in porcine tissues,and then the probe of NOBOX gene was generated and in-situ hybridization were used to detect NOBOX gene localization in porcine ovary.The results showed that NOBOX gene expression was significantly higher in ovary than in other tissues (P<0.01).There was no significant difference in NOBOX gene expression among kidney,liver,mammary gland,muscle and adrenal (P>0.05).In ovary,NOBOX gene was localized in oocyte cytoplasm specifically,but not in granulosa cell.All these results revealed that NOBOX gene was highly expressed in the cytoplasm of procine oocyte,indicating that it played an important role in porcine oocyte and early embryo development.

Key words: porcine; ovary; NOBOX gene; expression; in-situ hybridization

CLC Number:

Q786

YANG Yang, ZHANG Kai-xin, LIN Ye, LIN Lin, XING Wei-nan, NI Hua, HOU Xiao-ming. Study on Expression of Newborn Ovary Homeobox Gene in Porcine Ovary[J]. , 2015, 42(10): 2701-2706.

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Study on Expression of Newborn Ovary Homeobox Gene in Porcine Ovary

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