氢化物发生—原子荧光光谱法测定牛肉中的硒含量

doi:10.16431/j.cnki.1671-7236.2015.09.028

Abstract

Abstract: In this assay, we detected the selenium content in beef by hydride atomic fluorescence spectrometry.The conditions of the test were optimized by the selections of clearing up solution, pre-reducing agent, medium acid and so on, the limits of detection, precision and the standard addition recovery were determined.The results showed that 2:1 mixed acid as the clearing up solution, 6 mol/L hydrochloric acid as pre-reducing agent and the volume ratio of 10% hydrochloric acid as acid medium were chosen, the calibration curve was linear in the range from 0 to 8.0 μg/L with correlation coefficient was 0.9999.The limit of detection (LOD) was 0.01 ng/mL.At three adding levels of 20, 30 and 40 μg/L for selenium, the recovery of beef was 94% to 104% with RSD of 3.80%.This method had the characteristics of simple and effective, it had important referential significance for the detection of selenium in meat.

Key words: selenium; beef; hydride atomic fluorescence spectrometry

CLC Number:

O657.31

LIU Li-na, XU Fa-ting, ZHAO Fang-hong, QIN Shun-yi, YAO Wan, LIU Jia, LI Yu-zhu. Detection of Selenium Content in Beef by Hydride Atomic Fluorescence Spectrometry[J]. , 2015, 42(9): 2399-2404.

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Detection of Selenium Content in Beef by Hydride Atomic Fluorescence Spectrometry

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