碱性成纤维细胞生长因子对牛骨骼肌卫星细胞增殖的影响

doi:10.16431/j.cnki.1671-7236.2015.07.021

Abstract

Abstract: To investigate the effect of basic fibroblast growth factor (bFGF) on the proliferation of bovine skeletal muscle satellite cells,bovine skeletal muscle satellite cells were isolated and cultured in the medium with bFGF,the growth and differentiation state of muscle satellite cells were observed,and the growth curve and EDU cell proliferation assay were conducted.The results showed that cell morphology of bovine skeletal muscle satellite cells cultured in medium containing bFGF was better and proliferation rate was significantly higher than that in control group (P<0.05).The results indicated that bFGF could promote proliferation of bovine skeletal muscle satellite cells efficiently in growth medium and differential medium.Our study established an efficient method to culture bovine skeletal muscle satellite cells,which could provide reference for studying and using of skeletal muscle satellite cells.

Key words: bovine; skeletal muscle satellite cells; basic fibroblast growth factor; cell proliferation

CLC Number:

S823

ZHANG Wei-ran, DAI Yang, WANG Yi-min, LIU Xin-feng, WANG Ting, GUO Hong, DING Xiang-bin. Effect of Basic Fibroblast Growth Factor on the Proliferation of Bovine Skeletal Muscle Satellite Cells[J]. , 2015, 42(7): 1763-1769.

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Effect of Basic Fibroblast Growth Factor on the Proliferation of Bovine Skeletal Muscle Satellite Cells

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