2011年—2014年广西猪流行性腹泻病毒检测及其M基因的序列分析

doi:10.11843/j.issn.1671-7236.2015.03.007

Abstract

Abstract: 331 piglets diarrhea fecal samples,collected from Nanning,Yulin and other 12 cites in Guangxi from January 2011 to April 2014,were used to detect porcine epidemic diarrhea virus (PEDV) by RT-PCR method.210 samples were PEDV positive,the positive rate was 63.44%.25 PEDV M genes were cloned from the PEDV positive samples.25 M genes were sequenced,analyzed and compared with 51 PEDV reference strains which were published in GenBank,and the phylogenetic tree was drew.The nucleotide and amino acid homologies of M gene were 96.0% to 99.9% and 94.3% to 99.6%,respectively.The genetic variation analysis of M gene showed that the PEDV prevalent strains of Guangxi province in 2013 to 2014 were genetically close to the prevalent strains of Beijing,Anhui,Wuhan,Hebei,Guangdong and other places of China from 2010 to 2013.But there were genetically different from the early Chinese isolates CH/S (GenBank No.JN547228),the vaccine strains CV777 (GenBank No.AF353511) and Attenuated DR13 (GenBank No.JQ023162).The results suggested that in recent years Guangxi PEDV epidemic strains had obvious variations compared with the early Chinese PEDV isolates.

Key words: porcine epidemic diarrhea virus (PEDV); M gene; genetic variation

CLC Number:

LU Bing-xia, QIN Yi-bin, HE Ying, LI Ying-ying, LIANG Jia-xing, LI Ke-yu, LI Bin, SU Qian-lian, ZHOU Ying-ning, JIANG Dong-fu, LU Jing-zhuan, BI Bing-fen, LIANG Bao-zhong, DUAN Qun-peng, ZHAO Wu. Detection of Porcine Epidemic Diarrhea Virus from 2011 to 2014 in Guangxi Provine and Sequence Analysis of their M Genes[J]. , 2015, 42(3): 549-557.

References

[1] Debouck P,Pensaert M.Experimental infection of pigs with a new porcine enteric coronavirus,CV777[J].Am J Vet Res,1980,41(2):219-223.

[2] Debouck P,Pensaert M,Coussement W.The pathogenesis of an enteric infection in pigs,experimentally induced by coronavirus-like agent,CV777[J].Vet Microbiol,1981,6(2):157-165.

[3] 林初文,庄金秋,谢印乾.我国主要猪病毒性腹泻疾病的特点及其防治对策[J].中国畜牧兽医,2007,34(5):121-123.

[4] Chasey D,Cartwright S F.Virus-like particles associated with porcine epidemic diarrhea[J].Res Vet Sci,1978,25(2):255-256.

[5] Pensaert M B,DeBouck P.A new coronavirus-like particles associated with diarrhea in swine[J].Arch Virol,1978,58:243-247.

[6] Sueyoshi M,Tsuda T,Yamazaki K,et al.An immunohistochemical investigation of porcine epidemic diarrhea[J].J Comp Pathol,1995,113(1):59-67.

[7] Xuan H,Xing D,Wang D,et al.Study on the culture of porcine epidemic diarrhea virus adapted to fetal porcine intestine primary cell monolayer[J].Chin J Vet Sci,1984,4:202-208.

[8] 田小艳,孙华,邓雨修,等.3种致猪腹泻病毒的多重RT-PCR检测[J].动物医学进展,2009,30(9):54-57.

[9] 杨敏.猪流行性腹泻病毒结构蛋白基因的克隆及特异性分析[D].兰州:甘肃农业大学,2006.

[10] 张红垒,董洁,梁亚冰,等.猪流行性腹泻病毒的RT-PCR鉴定及其M、N和E基因的序列分析[J].西北农业学报,2012,21(9):24-28.

[11] 王隆柏,吴学敏,车勇良,等.猪流行性腹泻病毒ORF3和M基因的克隆与序列分析[J].中国动物传染病学报,2012,20(3):67-72.

[12] Sun R,Cai R,Chen Y,et al.Outbreak of porcine epidemic diarrhea in suckling piglets,China[J].Emerg Infect Dis,2012,18:161.

[13] 杜坚,蒋玉雯,白安斌,等.广西猪流行性腹泻流行病学调查[J].动物科学与动物医学,2004,6:52-53.

[14] 甘海霞,梁晟,韦显凯,等.2011年广西猪群猪流行性腹泻和猪传染性胃肠炎调查[J].动物医学进展,2012,33(10):125-127.

[15] 郭旋,陈静,刘欢,等.广西仔猪腹泻病毒病原流行病学调查[J].南方农业学报,2013,44(1):155-160.

Detection of Porcine Epidemic Diarrhea Virus from 2011 to 2014 in Guangxi Provine and Sequence Analysis of their M Genes

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