运输应激对驴血液指标的影响

doi:10.16431/j.cnki.1671-7236.2019.02.006

摘要/Abstract

摘要：

本试验旨在研究长途运输应激对驴血液指标的影响。试验以12头体重（140.8±5.2）kg的德州驴为研究对象，运输距离约950 km，发车地气温为-20℃，目的地气温为-10℃，历时21 h，其中包括10次停车观察（≤ 20 min）。分别在运输前、运输后0 h和运输后3、5、7、9、15 d采集颈静脉血15 mL，进行血液激素指标、血常规指标和血液生化指标检测，并分别记录运输前、运输后0 h和运输后10、20、30 d体重。结果显示，与运输前相比，落地后0 h血清中促肾上腺皮质激素（ACTH）、热休克蛋白90（HSP90）和皮质醇（cortisol）浓度均显著升高（P<0.05），落地后3、5、7、9、15 d内逐渐降低，ACTH和HSP90基本恢复至运输前水平；中性粒细胞总数（Neu）、红细胞总数（RBC）和血红蛋白浓度（HGB）均较运输前显著上升（P<0.05），白细胞总数（WBC）有所上升但与运输前差异不显著（P>0.05），淋巴细胞总数（LYM）较运输前显著下降（P<0.05）；血清总蛋白（TP）、血清白蛋白（ALB）、肌酐（CREA）、乳酸脱氢酶（LDH）、运输前后均未发生显著变化（P>0.05），谷草转氨酶（AST）、肌酸激酶（CK）、尿素（UREA）均较运输前显著上升（P<0.05）；体重较运输前显著下降（P<0.05），运输后10 d内体重已恢复至运输前水平。上述结果表明，长途运输可诱发驴的应激反应、肝脏损伤、机体代谢水平升高等，但免疫系统未受到明显损害，在落地后15 d内机体逐渐从应激反应中恢复至运输前状态。

关键词: 驴; 运输; 应激; 血液指标

Abstract:

This study was designed to investigate the effects of long-distance transport stress on blood parameters of donkey.The study was conducted with 12 heads of Dezhou donkeys with weight of (140.8±5.2) kg,the transportation distance was about 950 km,the departure temperature was -20℃,and the destination temperature was -10℃,which lasted for 21 h,including 10 parking observations (≤ 20 min).15 mL of jugular venous blood was collected before loading,0 h after transportation and 3,5,7,9 and 15 d after transportation,and blood hormone indexes,blood routine indexes and blood biochemical indexes were detected,and the weights before transport,0 h after transport and 10,20 and 30 d after transport were recorded.The results showed that the concentrations of adrenocorticotropic hormone (ACTH),heat shock protein 90 (HSP90) and cortisol in the serum at 0 h after landing were significantly higher than those before transportation (P<0.05),and gradually decreased within 3,5,7,9 and 15 d after transportation,and ACTH and HSP90 recovered to pre-carriage levels.The number of neutrophils (Neu),the total number of red blood cells (RBC) and the hemoglobin concentration (HGB) increased significantly after 3,5,7,9 and 15 d (P<0.05),the total number of white blood cells (WBC) increased but not significantly different from that before transportation (P>0.05),the total number of lymphocyte (LYM) was significantly lower than before transport (P<0.05).There were no significant changes in serum total protein (TP),serum albumin (ALB),creatinine (CREA) and lactate dehydrogenase (LDH) before and after transport (P>0.05).Aspartate aminotransferase (AST),creatine kinase (CK) and urea (UREA) were significantly higher than before transport (P<0.05).The body weight decreased significantly (P<0.05),and recovered to the pre-transport level within 10 d after transportation.The above results indicated that long-distance transport induced stress response to sputum,liver damage and elevated levels of metabolism,but the immune system was not significantly damaged.Within 15 d after landing,the body gradually recovered from the stress response to the pre-shipment state.

Key words: donkey; transport; stress; haemotological indices

中图分类号:

S822

高维平, 姜桂苗, 梁伟超, 李俊桥, 嵇传良, 赵付伟, 邓立新. 运输应激对驴血液指标的影响[J]. 《中国畜牧兽医》, 2019, 46(2): 373-379.

GAO Weiping, JIANG Guimiao, LIANG Weichao, LI Junqiao, JI Chuanliang, ZHAO Fuwei, DENG Lixin. Effect of Transport Stress on Haemotological Indices of Donkey[J]. , 2019, 46(2): 373-379.

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