油橄榄叶提取物对酒精性脂肪肝大鼠PP1-DNA-PK-USF1信号通路的干预作用

doi:10.16431/j.cnki.1671-7236.2018.08.035

摘要/Abstract

摘要：

试验旨在探讨油橄榄叶提取物（olive leaf extract，OLE）防治酒精性脂肪肝（AFLD）的作用机制。将50只健康大鼠随机均分为5组：空白对照组，模型组，OLE高、中、低剂量组，除空白对照组外，其他各组均采用递增法灌胃食用乙醇24周，建立大鼠肝损伤模型，造模同时OLE高、中、低剂量组分别用OLE（1 000、500、250 mg/kg）进行灌胃治疗。利用生物显微技术观察肝脏组织结构的变化，全自动生化分析仪测定血脂水平，实时荧光定量PCR、Western blotting和酶联免疫吸附法（ELISA）检测肝组织蛋白磷酸酶1（PP1）、DNA依赖的蛋白激酶（DNA-PK）、上游刺激因子1（USF1）mRNA水平和蛋白含量。结果显示，与空白对照组相比，模型组大鼠肝脏损伤程度明显加重，血清甘油三酯（TG）、游离脂肪酸（FFA）含量极显著升高（P<0.01）；肝脏PP1、DNA-PK、USF1水平和蛋白含量极显著升高（P<0.01）。与模型组相比，OLE干预后，肝脏病理改变明显减轻；血清TG、FFA含量显著或极显著降低（P<0.05；P<0.01）；肝脏PP1、DNA-PK、USF1 mRNA水平和蛋白含量显著或极显著降低（P<0.05；P<0.01）。结果表明，OLE可通过调节PP1-DNA-PK-USF1信号通路缓解肝脏脂肪变性，抑制AFLD的发展。

关键词: 油橄榄叶提取物; 酒精性脂肪肝(AFLD); 信号通路; 大鼠

Abstract:

To explore the mechanism of olive leaf extract (OLE) on alcohol hepatic injury,fifty rats were randomly divided into five groups,including blank control group,model group and three different doses groups of OLE.Hepatic injury models of rats were established by using edible alcohol with increasing dosage for 24 weeks.In the process of experimental hepatic injury,the rats in OLE groups were given OLE at doses of 1 000,500 or 250 mg/kg by intragastric administration,respectively.The changes of liver histological structure were observed by bio-microscopy.The levels of serum lipids were determined by automatic biochemistry analyzer.Real-time PCR,Western blotting and ELISA were used to detect mRNA and protein content changes of hepatic protein phosphatase 1 (PP1),DNA-dependent protein kinase (DNA-PK) and upstream stimulating factor 1 (USF1).The results showed that compared with blank control group,hepatic injury in the model group exhibited significant changes,the levels of serum triglyceride (TG) and free fatty acid (FFA) were extremely significantly increased (P<0.01),mRNA levels and protein contents of PP1,DNA-PK and USF1 in liver were extremely significantly increased (P<0.01).Compared with the model group,hepatic pathological changes were meliorated.OLE significantly or extremely significantly reduced the levels of serum TG and FFA (P<0.05;P<0.01),and mRNA levels and protein contents of PP1,DNA-PK and USF1 in liver (P<0.05;P<0.01).The results indicated that OLE could improve relieve fatty degeneration,and prevent the occurrence and development of alcohol hepatic injury by regulation of PP1-DNA-PK-USF1 signaling pathway.

Key words: olive leaf extract; alcoholic fatty liver disease (AFLD); signaling pathway; rat

中图分类号:

R285

王昱, 何九军. 油橄榄叶提取物对酒精性脂肪肝大鼠PP1-DNA-PK-USF1信号通路的干预作用[J]. 《中国畜牧兽医》, 2018, 45(8): 2327-2333.

WANG Yu, HE Jiujun. Intervention Effect of Olive Leaf Extract on PP1-DNA-PK-USF1 Signaling Pathway of Alcoholic Fatty Liver Disease Rats[J]. , 2018, 45(8): 2327-2333.

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