猪繁殖与呼吸综合征病毒(PRRSV)协同感染细菌性疾病的分析研究

doi:10.16431/j.cnki.1671-7236.2018.03.028

摘要/Abstract

摘要：

为了解湖北某养殖场猪繁殖与呼吸综合征病毒（porcine reproductive and respiratory syndrome virus，PRRSV）流行毒株的遗传变异和临床感染情况，试验采集10份疑似PRRS发病仔猪的肺脏、淋巴结等临床样品，应用RT-PCR方法扩增PRRSV的Nsp2部分基因用于定性检测分析，并对扩增的其中2份PRRSV阳性样品进行ORF5基因核苷酸序列测定，结合不同疫苗毒株开展同源性比对分析。为进一步揭示病因，通过多重PCR方法对10份发病猪的肺脏和12份鼻拭子样品进行了相关致病菌的鉴定，并对其中的2株不同病原菌开展药敏试验。结果显示，10份临床样品中有5份检测到美洲型变异PRRSV，病原阳性率为50%。ORF5全基因序列分析表明，2个流行毒株间的核苷酸同源性为99.7%，与以TJM-F92、JXA1-R、HuN4-F112等为代表的高致病性致弱疫苗毒株核苷酸同源性最高，为96.7%~97.0%；与美洲型标准毒株VR2332的同源性分别为87.6%和87.9%；与国内较早分离的经典毒株（CH-1R和R98株）的核苷酸同源性分别为92.9%和87.4%、87.7%。患病猪临床常见感染模式为PRRSV+PM+SS、PRRSV+PM或PRRSV+HPS，2株主要致病菌药敏试验表明，多杀性巴氏杆菌对头孢曲松、阿莫西林等药物高度敏感，猪链球菌对阿莫西林、氨苄西林、阿奇霉素等药物高度敏感。本研究揭示了该场保育猪的发病病原，并从分子水平明确了临床PRRSV与不同疫苗毒株的亲缘关系，为弱毒疫苗的合理选择使用和综合防控PRRS提供了实践依据。

关键词: 猪繁殖与呼吸综合征病毒(PRRSV); 细菌性疾病; 协同感染; 防控

Abstract:

To investigate the genetic variation and clinical infections of porcine reproductive and respiratory syndrome virus (PRRSV) pandemic strain in Hubei province,we collected 10 clinical samples (lung,lymph nodes) which were suspected infected with PRRSV,samples were detected using RT-PCR amplification of PRRSV Nsp2 gene.Futhermore,the nucleotide sequence of ORF5 gene from two PRRSV positive samples were determined,and homology comparison analysis based on Nsp2 were performed.Multiple PCR were used to detect particular pathogenic from 10 copies of pig lung disease and 12 copies of the associated nasal swab samples.The results showed that five copies in 10 clinical samples were identified as American type variable PRRSV,positive rate was 50%.ORF5 gene sequence analysis showed that all the nucleotide homology between two pandemic strain was 99.7%,and showed a highly nucleotide homology with TJM-F92,JXA1-R,HuN4-F112 about 96.7% to 97.0%;The homology rate with American standard strain VR2332 was 87.6% and 87.9%,respectively;The homology rate with classical strains of the separation of the earlier domestic (CH-1R and R98 strains) was 92.9% and 87.4%,87.7%,respectively.The main clinical common infection pattern were PRRSV+PM+SS,PRRSV+PM or PRRSV+HPS.Bacteria drug sensitive test results showed that the multiple kill pasteurella was highly sensitive against ceftriaxone,amoxicillin,etc,while Streptococcus suis was highly sensitive against amoxicillin,ampicillin,azithromycin,etc.This study revealed the clinical PRRSV strains in particular farms in Hubei province.The genetic relationship between clinical PRRSV strains and vaccine strains were also revealed.These results would provide theoretical basis for the development of new vaccines and drugs.

Key words: porcine reproductive and respiratory syndrome virus (PRRSV); bacterial disease; co-infection; prevention and control

中图分类号:

S852.65⁺9.6

李明波, 库旭钢, 赵海忠, 宋忠旭, 孙华, 梅书棋. 猪繁殖与呼吸综合征病毒(PRRSV)协同感染细菌性疾病的分析研究[J]. 《中国畜牧兽医》, 2018, 45(3): 781-789.

LI Mingbo, KU Xugang, ZHAO Haizhong, SONG Zhongxu, SUN Hua, MEI Shuqi. Study on Co-infection Analysis of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Bacterial[J]. , 2018, 45(3): 781-789.

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