基于皮肤组织转录组数据研究水牛与黄牛温度适应性的差异

doi:10.16431/j.cnki.1671-7236.2017.07.002

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (7): 1906-1914.doi: 10.16431/j.cnki.1671-7236.2017.07.002

基于皮肤组织转录组数据研究水牛与黄牛温度适应性的差异

齐昱¹, 邢燕平¹, 潘静¹, 凌宇¹, 曹宇¹, 孟凡华¹, 荆文魁²

1. 内蒙古农业大学生命科学学院, 呼和浩特 010019;
2. 中华人民共和国二连浩特出入境检验检疫局, 二连浩特 011100

收稿日期:2016-12-26 出版日期:2017-07-20 发布日期:2017-07-22
通讯作者: 孟凡华 E-mail:fhmeng2013@126.com
作者简介:齐昱(1987-),女,内蒙古通辽人,博士生,研究方向:动物细胞发育与调控,E-mail:wsqiyu@aliyun.com
基金资助:
内蒙古自然科学基金（2016MS0349）；内蒙古高等学校科学研究项目（NJZY16067）

Analysis of the Differences in Temperature Adaptability Between Buffaloes and Yellow Cattle Based on Transcriptome Data of Skin Tissues

QI Yu¹, XING Yan-ping¹, PAN Jing¹, LING Yu¹, CAO Yu¹, MENG Fan-hua¹, XING Wen-kui²

1. College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010019, China;
2. The People's Republic of China Erlianhot Entry Exit Inspection and Quarantine Bureau, Erlianhot 011100, China

Received:2016-12-26 Online:2017-07-20 Published:2017-07-22

摘要/Abstract

摘要：

为了找到与水牛和黄牛温度适应性差异相关的信号通路，本研究对3头成年水牛和3头成年黄牛的皮肤组织进行了转录组测序和生物信息学分析。结果显示，测序后水牛和黄牛皮肤组织分别得到59 369 167和69 837 009条reads，与参考基因组的唯一比对率分别为70.76%和75.55%。水牛和黄牛皮肤组织共有1 611个差异表达基因，其中801个在水牛皮肤中上调，810个在水牛皮肤中下调。GO分析结果显示，注释到细胞组分、分子功能、生物学过程的差异表达基因分别为316、263和278个。KEGG分析结果显示，差异表达基因共富集在33条信号通路上，其中神经活性配体-受体相互作用、氧化磷酸化、胆碱能突触、5-羟色胺能神经突触、GABA能突触、钙信号通路等信号通路可能与水牛和黄牛在温度适应性上的差异相关。综上所述，突触与氧化磷酸化可能与水牛和黄牛之间的温度适应性差异有关，本研究为进一步了解水牛和黄牛对温度适应性差异的分子机制和分子育种奠定基础。

关键词: 转录组; 信号通路; 差异表达基因; 皮肤; 水牛; 黄牛

Abstract:

In order to find out signaling pathways that related to the differences in temperature adaptability between buffaloes and Yellow cattle, RNA-sequencing and bioinformatics analysis were performed on the skin tissues of 3 adult buffaloes and 3 adult Yellow cattle. The results showed that 59 369 167 and 69 837 009 reads were obtained from buffaloes and Yellow cattle skin, respectively. The percentages of the uniquely mapped reads in buffaloes and Yellow cattle were 70.76% and 75.55%, respectively. There were 1 611 differentially expressed genes in buffaloes and Yellow cattle skin, 801 of which were up-regulated in buffaloes skin and 810 were down-regulated in buffaloes skin. GO analysis showed that there were 316, 263 and 278 differentially expressed genes classified to the cellular components, molecular functions and biological processes, respectively. KEGG analysis showed that the differentially expressed genes were enriched in 33 signaling pathways, some of which such as neuroactive ligand-receptor interaction, oxidative phosphorylation, cholinergic synapse, serotonergic synapse, GABAergic synapse and calcium signaling pathway might related to the differences in temperature adaptability between buffaloes and Yellow cattle. In summary, the synapse and oxidative phosphorylation might be related to the differences in temperature adaptability between buffalo and Yellow cattle. This study would lay a foundation for further understanding the molecular mechanism of temperature adaptation differences between buffaloes and Yellow cattle and molecular breeding.

Key words: transcriptome; signaling pathway; differentially expressed gene; skin; buffalo; Yellow cattle

中图分类号:

齐昱, 邢燕平, 潘静, 凌宇, 曹宇, 孟凡华, 荆文魁. 基于皮肤组织转录组数据研究水牛与黄牛温度适应性的差异[J]. 《中国畜牧兽医》, 2017, 44(7): 1906-1914.

QI Yu, XING Yan-ping, PAN Jing, LING Yu, CAO Yu, MENG Fan-hua, XING Wen-kui. Analysis of the Differences in Temperature Adaptability Between Buffaloes and Yellow Cattle Based on Transcriptome Data of Skin Tissues[J]. , 2017, 44(7): 1906-1914.

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基于皮肤组织转录组数据研究水牛与黄牛温度适应性的差异

Analysis of the Differences in Temperature Adaptability Between Buffaloes and Yellow Cattle Based on Transcriptome Data of Skin Tissues

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