连续培养瘤胃模拟发酵的工作参数设置研究进展

doi:10.16431/j.cnki.1671-7236.2017.01.015

摘要/Abstract

摘要：

体外培养法作为反刍动物营养学研究的重要方法，主要有批次培养法和连续培养法两种。由于连续培养法能够更好地模拟瘤胃内发酵情况，其应用也更为广泛。但连续培养瘤胃模拟装置的主要运行参数多，如温度、pH、稀释率、缓冲液与瘤胃液接种比、搅拌速率与方式等，且不同运行参数设置以及参数的组合对试验结果影响较大，因此作者在总结前人研究结果的基础上，就不同工作参数对连续培养瘤胃模拟发酵过程中挥发性脂肪酸、干物质消失率、pH、酶活、原虫数量、微生物蛋白等指标的影响进行了综述。

关键词: 体外培养; 连续培养系统; 运行参数

Abstract:

In vitro culture method is an important way to research in ruminant dietetics, including batch and continuous culture methods. Because the continuous culture method can better simulate the conditions of fermentation in rumen, it was applied more widely. There are many operation parameters of rumen simulation technique involved in continuous culture, such as temperature, pH, dilution rate, the proportion of buffer to rumen fluid, stirring rate and way, meanwhile, different operation parameters and their combinations will have a great influence on the fermentation results. On the basis of summarizing the results of previous studies, the authors review the effects of different parameters on volatile fatty acid, dry matter disappearance rate, pH, enzyme activity, number of protozoa and microbial protein, in dual-flow continuous culture system in this paper.

Key words: in vitro culture; continuous culture system; operating parameters

中图分类号:

Q482

伍梦楠, 沈维军, 张佩华, 陈东. 连续培养瘤胃模拟发酵的工作参数设置研究进展[J]. 《中国畜牧兽医》, 2017, 44(1): 106-112.

WU Meng-nan, SHEN Wei-jun, ZHANG Pei-hua, CHEN Dong. Research Progress on Working Parameters of the Continuous Culture of Rumen Simulation System[J]. , 2017, 44(1): 106-112.

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