肉羊微生物固态发酵饲料组方筛选及翻料工艺的研究

doi:10.16431/j.cnki.1671-7236.2016.09.013

《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (9): 2302-2309.doi: 10.16431/j.cnki.1671-7236.2016.09.013

肉羊微生物固态发酵饲料组方筛选及翻料工艺的研究

张立明, 郭鹏, 张政, 张棋炜, 刘大程

内蒙古农业大学兽医学院, 农业部动物疾病临床诊疗技术重点实验室, 呼和浩特 010018

收稿日期:2016-03-11 出版日期:2016-09-20 发布日期:2016-09-20
通讯作者: 刘大程 E-mail:nmgldc@163.com
作者简介:张立明(1990-),男,山西孝义人,硕士生,研究方向:反刍动物瘤胃微生态,E-mail:1401307372@qq.com
基金资助:
国家自然科学基金项目（31260560）；现代农业（奶牛）产业技术体系建设专项（CARS-37）；内蒙古科技厅专利转化项目（20140185）；内蒙古农业大学科技成果转化项目（CGZH2013007）

Study on Formula Screening and Mixing Technology of Microbial Solid-state Fermentation Feed for Mutton Sheep

ZHANG Li-ming, GUO Peng, ZHANG Zheng, ZHANG Qi-wei, LIU Da-cheng

Key Laboratory of Animal Disease Clinical Diagnosis Technology, Ministry Department of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China

Received:2016-03-11 Online:2016-09-20 Published:2016-09-20

摘要/Abstract

摘要：

本试验旨在研制一种肉羊微生物发酵饲料，并确定其固态翻料工艺。以酿酒酵母菌BC、XR4与枯草芽孢杆菌A15为协同发酵菌株；选取麦麸、米糠、枣渣等为发酵饲料原辅料，采用DPS软件对原料进行均匀混料试验设计，选取8个组方进行固态发酵试验，通过测定发酵料中的活菌数，β-葡聚糖、甘露聚糖、多肽等营养活性物质的含量来筛选最优的组方。同时，对筛选出的组方进行翻料工艺的研究，其中，对照组不翻料，试验1组翻料1次，试验2组翻料2次，通过测定发酵过程中的料温变化、发酵料活菌数及3种营养活性物质的含量来确定翻料工艺参数。结果显示，8个试验组中的活菌数均呈现先上升然后快速下降的趋势，3种营养活性物质含量随着发酵时间的延长而显著升高（P<0.05），其中组方7的综合指标最优，其活菌数最高达到了37.30×10⁵ CFU/g，β-葡聚糖、甘露聚糖、多肽含量分别为97.41 mg/100 mg、37.66 mg/100 mg、20.17 μg/100 mg；翻料工艺试验中，发酵结束时2个试验组的活菌数和3种营养活性物质均显著高于对照组（P<0.05），试验2组显著高于试验1组（P<0.05），试验2组的活菌数最高值、β-葡聚糖、甘露聚糖、多肽含量分别比对照组提高10.1%、7.5%、7.6%、3.0%。

关键词: 肉羊; 微生物固态发酵饲料; 饲料组方; 翻料工艺

Abstract:

The experiment was designed to develop a microorganism fermentation feed for mutton sheep,and determined its solid-state mixing technology.In this experiment,the wheat bran,rice bran and jujube fruit residues were chosen as the solid-state fermentation media,and combined the Saccharomyces cerevisiae strains BC,XR4 and Bacillus subtilis strain A15 as the fermentation strains.The DPS software was applied to design the uniform mixture experiment,and 8 formulas were selected for solid-state fermentation experiments,and evaluated the optimal formula by measuring the number of live bacterium and three kinds of nutritional active substances (β-glucan,mannan and peptide).Meanwhile,we studied the mixing technology on the selected formula,and the control group was without turned mixing,the experimental group 1 was turned mixing once and the experimental group 2 was turned mixing twice.The optimal mixing technology was determined by monitoring the feed temperature,the number of live bacterium and the content of three kinds of nutritional active substances (β-glucan,mannan and peptides) during the solid-state fermentation.The results showed that the number of live bacterium of 8 formulas all increased first and then decreased rapidly.Three kinds of nutritional active substances were significantly increased with the extension of fermentation (P<0.05).The formula 7 had the optimal comprehensive index,of which the living bacterium number reached 37.30×10⁵ CFU/g and the contents of three nutrient active substances were 97.41 mg/100 mg,37.66 mg/100 mg and 20.17 μg/100 mg.In the mixing technology experiment,the number of live bacterium and nutritional active substances of the experimental groups were significantly higher than the control group (P<0.05).Comparing with the control group,the number of live bacterium and the content of nutritional active substances in experimental group 2 increased by 10.1%,7.5%,7.6%,3.0%,respectively.

Key words: mutton sheep; microbial solid-state fermentation feed; feed formula; material mixing technology

中图分类号:

S816

张立明, 郭鹏, 张政, 张棋炜, 刘大程. 肉羊微生物固态发酵饲料组方筛选及翻料工艺的研究[J]. 《中国畜牧兽医》, 2016, 43(9): 2302-2309.

ZHANG Li-ming, GUO Peng, ZHANG Zheng, ZHANG Qi-wei, LIU Da-cheng. Study on Formula Screening and Mixing Technology of Microbial Solid-state Fermentation Feed for Mutton Sheep[J]. , 2016, 43(9): 2302-2309.

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肉羊微生物固态发酵饲料组方筛选及翻料工艺的研究

Study on Formula Screening and Mixing Technology of Microbial Solid-state Fermentation Feed for Mutton Sheep

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