Masson三色染色与HE染色在豚鼠牙齿组织石蜡切片中的比较研究及应用

›› 2011, Vol. 38 ›› Issue (3): 55-57.

Masson三色染色与HE染色在豚鼠牙齿组织石蜡切片中的比较研究及应用

韩天龙¹，王敏¹，红敏¹，萨其仍贵¹，查干其其格²，和希格¹，孙德欣¹

（1.赤峰市农牧科学研究院，内蒙古赤峰 024031； 2. 内蒙古国际蒙医医院，内蒙古呼和浩特 010010）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-03-20 发布日期:2011-03-20

he Comparison and Application of Masson Trichrome and HE Staining in Histological Paraffin Sections of Teeth in Guinea Pig

HAN Tian-long¹，WANG Min¹，HONG Min¹，SA Qi-renggui¹，CHA Gan-qiqige²，HE Xi-ge¹，SUN De-xin¹

（1. Chifeng Academy of Agriculture and Animal Husbandry Sciences, Chifeng 024031, China; 2. Inner Mongolia International Hospital of Mongolian Medicine, Hohhot 010010, China）

Received:1900-01-01 Revised:1900-01-01 Online:2011-03-20 Published:2011-03-20

摘要/Abstract

摘要： 针对牙齿组织形态学研究的难点，选择适宜固定液，用EDTA-2Na进行脱钙，完成了牙齿组织石蜡切片的制作。通过确定染色程序及各种染料的最佳作用时间，分别利用Masson三色染色法、传统的苏木精和伊红（HE）染色法对牙齿组织切片进行了染色观察。染色结果表明，与HE染色法相比，Masson三色染色更能使牙齿的各主要成分以不同颜色显现出来，色彩对比鲜明，亮丽润眼，为牙齿组织生理及病理组织形态学研究奠定了基础。针对机体中硬组织的相似特性，该试验方法可以在骨骼、软骨等硬组织的形态学研究中参考、改进并推广使用。

关键词: HE染色; Masson三色染色法; 石蜡切片; 牙齿组织; 脱钙; 胶原纤维

Abstract: In view of the difficulty of teeth of morphological study, chose the proper reagent for fixed, and the appropriate decalcify solution （EDTA-2Na） for completing the paraffin sections of teeth. The teeth histological sections were carried out using Masson trichrome staining and HE staining, by determining the advisable procedure and staining time firstly. Compared with HE staining, the main components were stained with different colours for bright and contrasted more obvious in Masson trichrome staining, which would paly an important role on the physiology and pathological morphological studies of teeth. For the similar characteristics of hard tissues in the body, this method could be referenced, improved and applied in the bone, cartilage and other hard tissues.

Key words: HE staining; Masson trichrome staining; paraffin section; dental tissues; decalcification; collagen fibers

中图分类号:

S852.16⁺5

韩天龙;王敏;红敏;萨其仍贵;查干其其格;和希格;孙德欣. Masson三色染色与HE染色在豚鼠牙齿组织石蜡切片中的比较研究及应用[J]. , 2011, 38(3): 55-57.

HAN Tian-long;WANG Min;HONG Min;SA Qi-renggui;CHA Gan-qiqige;HE Xi-ge;SUN De-xin. he Comparison and Application of Masson Trichrome and HE Staining in Histological Paraffin Sections of Teeth in Guinea Pig[J]. , 2011, 38(3): 55-57.

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