广西南宁市水牛肝脏内前后盘吸虫的鉴定

doi:10.16431/j.cnki.1671-7236.2021.02.029

摘要/Abstract

摘要： 为探明广西南宁地区水牛体内前后盘吸虫的种类，通过对其肝脏来源虫体进行鉴定，确定水牛感染的具体种属。本研究对南宁市某屠宰场水牛肝脏进行剖检、病理观察，并采集肝脏内寄生的前后盘吸虫进行虫卵孵育，对孵育所得的虫卵及虫体进行形态观察，对成虫虫体进行HE及苏木素染色，观察其内部的结构，并采用分子生物学方法对成虫进行鉴定。结果显示，虫体感染的肝脏表面有出血点，且呈现淤血、化脓灶、肝脏肿大、质地偏软等肉眼可见病变。虫卵呈椭圆形、淡灰色、有卵盖且内含圆形胚细胞，卵黄细胞不充满整个虫卵。对成虫染色观察可见虫体由体壁及内部生殖、消化等系统构成。体壁由皮层和肌层组成，虫体可见生殖系统、消化管和一些肌束与纤维组织，其中生殖系统包括卵巢、子宫和睾丸等生殖器官，消化管分布于整个体腔。虫体核糖体基因ITS-2扩增产物为441 bp，分析显示与GenBank中Explanatum explanatum（AB743577.1）相似性达99.3%。遗传进化分析显示此虫体与Explanatum explanatum具有很近的亲缘关系，同属一个分支。综合剖检病理变化，虫卵特性，虫体形态特性及分子生物学结果，确定该虫体为前后盘科的Explanatum explanatum。本研究结果为前后盘科吸虫分类鉴定提供一定的理论基础，并进一步丰富该科的虫体种类，此外初步了解广西南宁地区水牛前后盘吸虫病的流行情况，并为准确防治吸虫感染提供一定的理论依据。

关键词: 虫卵形态; HE染色; 形态学鉴定; 分子生物学鉴定; 前后盘吸虫

Abstract: To identify the accurate species of Paramphistomidae in buffalos of Nanning,Guangxi,trematode identification was performed after collected from livers.In this study,the liver of buffaloes in a slaughterhouse in Nanning was dissected and the pathological lesion was recorded.Afterwards,adult worms in the livers were collected and incubated to lay eggs,then morphology of the adult worms and eggs were observed.The adult worms were also stained with hematoxylin-eosin (HE) and hematoxylin to facilitate its internal structure observation.Finaly,adult worms were identified by molecular biological method.The results showed that there were bleeding spots on the infected liver surface.Besides,congestion,purulent focus,liver enlargement and softer texture was also detectable.The eggs were oval,light gray,covered with round embryo cells,and the yolk cells did not fill the whole egg.The adult was composed of body wall,internal reproductive and digestive system.The body wall was composed of cortex and muscular layer.The reproductive system,digestive tract and some muscle bundles and fibrous tissues also could be seen.The reproductive system included the reproductive organs such as ovary,uterus and testis,while the digestive tract was distributed throughout the body cavity.The amplified product of ITS-2 gene was 441 bp,and comparison with Explanatum explanatum (AB743577.1) in GenBank showed that the similarity was 99.3%.Phylogenetic analysis showed its close relationship with Explanatum explanatum,and they were also located in the same branch.Considering the pathological lesion,egg characteristics,adult morphological and molecular biology characteristics,flukes here in our study was determined as Explanatum explanatum. The results of this study provided a theoretical basis for the classification and identification of trematodes,and further enrich the species of this family.In addition,we have a preliminary understanding of the prevalence of Paramphistomidae in buffaloes in Nanning,Guangxi,and provided a theoretical basis for the accurate control of trematode infection.

Key words: egg morphology; HE staining; morphological identification; molecular biological identification; Paramphistomidae

中图分类号:

S855.9

吴正姣, 侯林静, 饶国顺, 靳纬坤, 方芳, 张为宇, 邸文达. 广西南宁市水牛肝脏内前后盘吸虫的鉴定[J]. 中国畜牧兽医, 2021, 48(2): 668-675.

WU Zhengjiao, HOU Linjing, RAO Guoshun, JIN Weikun, FANG Fang, ZHANG Weiyu, DI Wenda. Identification of Paramphistomidae in the Liver of Buffalos in Nanning,Guangxi[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 668-675.

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