番鸭细小病毒浙江分离株VP基因的克隆与序列分析

doi:10.16431/j.cnki.1671-7236.2015.10.013

›› 2015, Vol. 42 ›› Issue (10): 2600-2605.doi: 10.16431/j.cnki.1671-7236.2015.10.013

番鸭细小病毒浙江分离株VP基因的克隆与序列分析

万春和^1,2, 陈红梅², 傅秋玲², 施少华², 傅光华², 程龙飞², 黄瑜², 胡开辉¹

1. 福建农林大学生命科学学院, 福州 350002;
2. 福建省农业科学院畜牧兽医研究所, 福建省畜禽疫病防治工程技术研究中心, 福州 350013

收稿日期:2015-03-11 出版日期:2015-10-20 发布日期:2015-10-23
通讯作者: 黄瑜, 胡开辉 E-mail:huangyu_815@163.com;2692609765@qq.com
作者简介:万春和(1982-),男,湖北鄂州人,博士生,助理研究员,研究方向:水禽病毒分子生物学,E-mail:chunhewan@126.com
基金资助:
现代农业产业体系建设专项资金(CARS-43);公益行业农业科研专项(201003012);福建省自然科学基金(2015J01114)

Cloning and Sequence Analysis of VP gene of Muscovy Duck Parvovirus Zhejiang Isolate

WAN Chun-he^1,2, CHEN Hong-mei², FU Qiu-ling², SHI Shao-hua², FU Guang-hua², CHENG Long-fei², HUANG Yu², HU Kai-hui¹

1. College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2. Fujian Animal Disease Control Technology Development Center, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China

Received:2015-03-11 Online:2015-10-20 Published:2015-10-23

摘要/Abstract

摘要： 为明确番鸭细小病毒结构蛋白VP的特征,本研究运用PCR从已分离鉴定的番鸭细小病毒浙江分离株(MDPV-ZJ)中分段扩增出VP基因,并对其进行克隆测序和分析。结果表明,番鸭细小病毒浙江分离株VP基因全长为2 199 bp,编码732个氨基酸。所编码的包膜蛋白大小为81.32 ku、理论等电点为6.59、不稳定系数为37.49、亲水性平均系数为－0.667,属于亲水性稳定类蛋白,该编码的结构蛋白没有信号肽,为非分泌蛋白。根据VP基因特征从遗传进化上可将MDPV分为2个大的基因型:经典型和基因重组型,经典型MDPV可以进一步划分为台湾亚群、大陆亚群和欧洲群,MDPV各分离株在遗传进化上存在明显的地域性。本试验中MDPV浙江分离株株处于MDPV大陆亚群。

关键词: 番鸭细小病毒; VP基因; 克隆; 序列分析

Abstract: In order to enrich the biological characteristics of VP gene from Muscovy duck parvovirus Zhejiang isolate (MDPV-ZJ),the target VP gene fragments were amplified by PCR method with specific primers,then the obtained PCR products were cloned and sequenced.The bioinformatics analysis of VP gene of MDPV-ZJ was conducted.The results revealed that MDPV-ZJ VP gene was 2 199 bp in length,coding an open reading frame (ORF) with 732 amino acids.The molecular weight,theoretical isoelectric point,instability index and grand average of hydropathicity of MDPV-ZJ VP gene were 81.32 ku,6.59,37.49 and －0.667,respectively,and with no signal peptide.The genetic evdution ary tree based on the VP gene showed that the MDPV isoaltes contains two groups:The typical MDPV and recombinant MDPV; Also,the typical MDPV could be divided into three branches:Taiwanese branch,Maniland China branch and Euro branch,which existed obvious regional genetic evolution relationship.In this assay MDPV-ZJ was belonged to MDPV Maniland China branch.

Key words: Muscovy duck parvovirus; VP gene; clone; sequence analysis

中图分类号:

万春和, 陈红梅, 傅秋玲, 施少华, 傅光华, 程龙飞, 黄瑜, 胡开辉. 番鸭细小病毒浙江分离株VP基因的克隆与序列分析[J]. , 2015, 42(10): 2600-2605.

WAN Chun-he, CHEN Hong-mei, FU Qiu-ling, SHI Shao-hua, FU Guang-hua, CHENG Long-fei, HUANG Yu, HU Kai-hui. Cloning and Sequence Analysis of VP gene of Muscovy Duck Parvovirus Zhejiang Isolate[J]. , 2015, 42(10): 2600-2605.

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番鸭细小病毒浙江分离株VP基因的克隆与序列分析

Cloning and Sequence Analysis of VP gene of Muscovy Duck Parvovirus Zhejiang Isolate

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