4种犬科动物黑素皮质激素受体1基因序列比对及遗传进化分析

中国畜牧兽医

4种犬科动物黑素皮质激素受体1基因序列比对及遗传进化分析

徐超,宋兴超,岳志刚,李光玉,赵蒙,赵伟刚,魏海军,赵家平

(中国农业科学院特产研究所，农业部特种经济动物遗传育种与繁殖重点实验室，吉林省特种经济动物分子生物学省部共建国家重点实验室,吉林长春 130112)

出版日期:2014-01-20 发布日期:2014-02-27
通讯作者: 赵家平(1973—)，男，吉林人，硕士生导师，副研究员，从事特种经济动物遗传育种研究。E-mail: tcszjp@126.com
作者简介:徐超(1985—)，男，山东人，硕士，研究实习员，研究方向：经济动物繁殖育种。
基金资助:
吉林市科技支撑计划(201222307)；吉林省科技支撑计划(20130206029NY)；公益性行业(农业)科研专项(200903014)。

Sequence Comparison and Genetic Evolution Analysis of MC1R Gene in Four Canidae Families

XU Chao, SONG Xing-chao, YUE Zhi-gang, LI Guang-yu, ZHAO Meng,ZHAO Wei-gang, WEI Hai-jun, ZHAO Jia-ping

(State Key Laboratory of Special Economic Animal Molecular Biology, Key Laboratory of Special Economic Animal Genetic Breeding and Reproduction, Ministry of Agriculture, Institute of Special Animal and Plant Science of CAAS, Changchun 130112, China)

Online:2014-01-20 Published:2014-02-27

摘要/Abstract

摘要： 基于GenBank中已公布的家犬(Canis familiaris)、赤狐(Vulpes vulpes)、北极狐(Alopex lagopus)和貉(Nyctereutes procyonoides)黑素皮质激素受体1(MC1R)基因序列，利用BioEdit 7.0等生物信息软件，对4种犬科动物MC1R基因进行序列比对和遗传进化分析。结果表明,家犬、赤狐、北极狐及貉MC1R基因为单一外显子，编码区序列长度均为954 bp，碱基组成表现为C＞G＞T＞A,且G+C百分含量高于A+T；编码区碱基序列中共检测到20个突变位点，包括16个单一突变和4个简约突变，转换类型多于颠换，核苷酸和氨基酸序列同源性较高；赤狐与北极狐间的遗传距离最短，邻近法(NJ)、最小进化法(ME)和非对组算数平均法(UPGMA)3种方法构建的进化树基本一致，赤狐与北极狐首先聚为一簇，貉比家犬、赤狐和北极狐分化时间更早。

关键词: 犬科动物; 黑素皮质激素受体1; 序列比对; 遗传进化

Abstract: Based on the MC1R nucleotide and amino acid sequences of Canis familiaris, Vulpes vulpes, Alopex lagopus and Nyctereutes procyonoides published in GenBank, sequence variation and genetic evolution of MC1R gene were analyzed by means of bioinformatics softwares. The results showed that， the length of Canidae family MC1R gene coding sequence was 954 bp, the base composition was C＞G＞T＞A and the content of G+C was higher than that of A+T; A total of 20 polymorphic sites, including 16 single mutation sites and 4 parsimony informative sites were detected from four Canidae familes. In addition, the base conversion was more than transversion types. There were higher similarity of nucleotide and amino acid between four Canidae families; The phylogenetic distance between the Vulpes vulpes and Alopex lagopus was shorter than that between Vulpes vulpes and Canis familiaris. The result was basically consistent in molecular phylogenetic tree constructed by three methods which were NJ, ME and UPGMA, the Vulpes vulpes was clustered together with Alopex lagopus, Nyctereutes procyonoides was earlier divergent than the other three Canidae families.

Key words: Canidae family; melanocortin-1 receptor; sequence comparison; genetic evolution

徐超,宋兴超,岳志刚,李光玉,赵蒙,赵伟刚,魏海军,赵家平. 4种犬科动物黑素皮质激素受体1基因序列比对及遗传进化分析[J]. 中国畜牧兽医.

XU Chao, SONG Xing-chao, YUE Zhi-gang, LI Guang-yu, ZHAO Meng,ZHAO Wei-gang, WEI Hai-jun, ZHAO Jia-ping. Sequence Comparison and Genetic Evolution Analysis of MC1R Gene in Four Canidae Families[J]. .

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