动物源产CMY-2大肠杆菌分子流行病学研究

›› 2013, Vol. 40 ›› Issue (10): 47-51.

动物源产CMY-2大肠杆菌分子流行病学研究

郭玉芳¹, 赵秋云¹, 姜芮¹, 任金程¹, 杨玲¹, 甄盼盼², 汤电³, 蒋红霞¹

1. 华南农业大学兽医学院, 广东省兽药研制与安全评价重点实验室, 广东广州 510642;
2. 河北新华科极兽药有限公司, 河北石家庄 050000;
3. 武汉科前动物生物制品有限责任公司, 湖北武汉 430000

收稿日期:2013-03-20 出版日期:2013-10-20 发布日期:2013-12-19
通讯作者: 蒋红霞 E-mail:hxjiang@scau.edu.cn
作者简介:郭玉芳(1986-),女,河北人,硕士生,研究方向:兽医药理与毒理学。
基金资助:
国家自然科学基金—广东基金联合基金重点项目(U1031004)。

Epidemiology Investigation of CMY-2-producing E.coli from Animals

GUO Yu-fang¹, ZHAO Qiu-yun¹, JIANG Rui¹, REN Jin-cheng¹, YANG Ling¹, ZHEN Pan-pan², TANG Dian³, JIANG Hong-xia¹

1. Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;
2. Hebei Xinhua Keji Animal Medicine Co., Ltd., Shijiazhuang 050000, China;
3. Wuhan Keqian Animal Biological Products Co., Ltd., Wuhan 430000, China

Received:2013-03-20 Online:2013-10-20 Published:2013-12-19

摘要/Abstract

摘要： 为调查产CMY-2大肠杆菌在广东各养殖场的流行情况,对2010—2011年间分离自猪、鸡、鸭、鹅等动物的1293株大肠杆菌,采用PCR方法筛选出bla_CMY-2阳性菌株,琼脂稀释法测定阳性菌株对17种抗微生物药物的敏感性;接合转移试验和XbaⅠ酶切PFGE图谱分析bla_CMY-2基因转移扩散的方式。结果显示,1293株大肠杆菌中27 株含有bla_CMY-2基因,检出率为2.09%,均为多重耐药菌株,主要耐药谱型为AMP/CHL/TET/FLF/CTF/CTX/CAZ/CTR/GEN/CIP/ENR/NAL/OQX;27 株携带bla_CMY-2基因菌株中有14 株的bla_CMY-2基因可随质粒转移到受体菌E.coli C600中,且往往与bla_TEM-1和(或)qnrS1共同转移;PFGE分析结果显示,27 株携带bla_CMY-2基因菌株共产生17条谱带,其中有4株菌株,两两分别来自同一地区,存在克隆传播关系。提示,在广东地区食品动物养殖场内存在产CMY-2大肠杆菌的克隆传播,且bla_CMY-2基因伴随可转移质粒或其他可转移移动元件可能是造成产CMY-2大肠杆菌流行分布的主要原因。

关键词: 大肠杆菌; 耐药性; 动物源; CMY-2

Abstract: To investigate the epidemiology of CMY-2-producing E.coli from different animals throughout Guangdong province, 1293 isolates from pig, chicken, duck and goose from 2010 to 2011 were recruited in this study. All the isolates were screened for bla_CMY-2 gene by PCR and sequenced; the MICs of bla_CMY-2-positive isolates to 17 kinds of antimicrobial drugs were tested by agar dilution; conjugation experiments were performed on bla_CMY-2 harboring isolates to explore the transferability of bla_CMY-2gene to E.coli C600, and XbaⅠ-PFGE analysis were used to determine the transfer and spread manner of bla_CMY-2 among E.coli isolates. The results showed that 27(2.09%)of 1293 isolates carried bla_CMY-2,and were present multi-resistant phenotypes, in which the main one was AMP/CHL/TET/FLF/CTF/CTX/CAZ/CTR/GEN/CIP/ENR/NAL/OQX. The bla_CMY-2 gene in 14 of 27 isolates transferred successfully to E.coli C600 by plasmids, and most of them co-transferred with bla_TEM-1 and/or qnrS1. PFGE profiles showed that 17 different patterns were successfully got, and two from the same place were clonally related as well as another two isolates. CMY-2 encoding gene on a transferability plasmid or carried by other mobile element presumably was the major cause for the epidemiology of CMY-2-producing E.coli among food animals.

Key words: E.coli; resistance; animals; CMY-2

中图分类号:

S852.61

郭玉芳, 赵秋云, 姜芮, 任金程, 杨玲, 甄盼盼, 汤电, 蒋红霞. 动物源产CMY-2大肠杆菌分子流行病学研究[J]. , 2013, 40(10): 47-51.

GUO Yu-fang, ZHAO Qiu-yun, JIANG Rui, REN Jin-cheng, YANG Ling, ZHEN Pan-pan, TANG Dian, JIANG Hong-xia. Epidemiology Investigation of CMY-2-producing E.coli from Animals[J]. , 2013, 40(10): 47-51.

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