猪繁殖与呼吸综合征病毒GX1001株和GX1002株全基因组分子特征分析

›› 2013, Vol. 40 ›› Issue (4): 1-9.

• 生物技术 • 下一篇

猪繁殖与呼吸综合征病毒GX1001株和GX1002株全基因组分子特征分析

林昌华¹, 施开创², 陶春爱³, 钟诚¹, 莫胜兰², 李刚³

1. 广西大学动物科学技术学院,广西南宁 530005;
2. 广西动物疫病预防控制中心,广西南宁 530001;
3. 中国农业科学院北京畜牧兽医研究所,北京 100193

收稿日期:2012-08-31 出版日期:2013-04-20 发布日期:2013-04-19
通讯作者: 施开创(1968-),男,广西人,博士,副研究员,主要从事动物疫病诊断与防控技术研究。E-mail:shikaichuang@126.com E-mail:shikaichuang@126.com
作者简介:林昌华(1986-),男,广西人,硕士生,主要从事动物疫病诊断与防控技术研究。
基金资助:
广西科学基金项目(桂科青0832057)。

Analysis of Genomic Characteristics of Porcine Reproductive and Respiratory Syndrome Virus GX1001 and GX1002 Strains Isolated in Guangxi Province

LIN Chang-hua¹, SHI Kai-chuang², TAO Chun-ai³, ZHONG Cheng¹, MO Sheng-lan², LI Gang³

1. College of Animal Science and Technology, Guangxi University, Nanning 530005,China;
2. Guangxi Center for Animal Disease Control and Prevention, Nanning 530001,China;
3. Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China

Received:2012-08-31 Online:2013-04-20 Published:2013-04-19

摘要/Abstract

摘要： 为了解广西地区猪繁殖与呼吸综合征病毒(PRRSV)的变异情况及分子遗传特征,采用RT-PCR分段扩增2010年PRRSV广西地方分离株GX1001株和GX1002株基因片段,经克隆、测序、拼接,获得2个分离株的全基因序列。结果显示,不包括Poly(A)尾,GX1001株、GX1002株基因组全长分别为15329和15318 bp。同源性分析结果表明,2个分离株间全基因组核苷酸序列同源性为99.3%,与国内外北美洲型代表毒株间的全基因组核苷酸序列同源性为84.9%~99.5%,与欧洲型代表毒株间的同源性为61.5%~61.9%。序列分析结果表明,2个分离株的NSP2编码区均存在第481和533-561位,共30个氨基酸的不连续缺失,具有PRRSV高致病性变异毒株(HP-PRRSV)的遗传特征,但分别在不同区域出现新的突变、缺失及插入等变异现象。遗传进化分析结果表明,所有美洲型毒株可分为4个亚群,GX1001株和GX1002株均属于以高致病性JXA1株为代表的第4亚群。上述结果表明,本研究获得的PRRSV广西地方分离株GX1001株和GX1002株均属于HP-PRRSV,但其基因组在不同区域发生了新的遗传变异现象。

关键词: 猪繁殖与呼吸综合征病毒; 高致病性分离株; 全基因组特征; 变异分析

Abstract: To investigate the genomic characteristics of porcine reproductive and respiratory syndrome virus (PRRSV), the full-length genome of PRRSV GX1001 and GX1002 strains from Guangxi province were amplified by RT-PCR, sequenced and analyzed. The sequence data showed that, excluding the poly (A) tail, the genomic sequences of GX1001 and GX1002 were 15329 and 15318 bp in length, respectively. Full-length sequence analysis revealed that GX1001 and GX1002 shared 99.3% nucleotide identity with each other, 84.9% to 99.5% nucleotide identity with representative North American (NA) type isolates, 61.5% to 61.9% nucleotide identity with representative European (EU) type isolates, respectively. Full-length sequence comparisons demonstrated that GX1001 and GX1002 had discontinuous 30 aa deletion in 481th aa and 533th to 561th aa of NSP2 region compared with that of the NA prototype VR-2332, which was considered as the genetic marker of highly pathogenic PRRSV(HP-PRRSV), and had new multiple genomic variations such as mutation, deletion and insertion distributing in different regions. Phylogenetic analysis showed that all the NA type isolates could be divided into four subgroups, and GX1001 and GX1002 belonged to subgroup 4 represented by the highly pathogenic JXA1 strain. The results indicated that GX1001 and GX1002 strains from Guangxi province belonged to HP-PRRSV with new characteristic variations in different regions. This study provided valuable genomic data for further surveillance of HP-PRRSV.

Key words: porcine reproductive and respiratory syndrome virus; highly pathogenic isolate; genomic characteristics; variation analysis

中图分类号:

Q781

林昌华, 施开创, 陶春爱, 钟诚, 莫胜兰, 李刚. 猪繁殖与呼吸综合征病毒GX1001株和GX1002株全基因组分子特征分析[J]. , 2013, 40(4): 1-9.

LIN Chang-hua, SHI Kai-chuang, TAO Chun-ai, ZHONG Cheng, MO Sheng-lan, LI Gang. Analysis of Genomic Characteristics of Porcine Reproductive and Respiratory Syndrome Virus GX1001 and GX1002 Strains Isolated in Guangxi Province[J]. , 2013, 40(4): 1-9.

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猪繁殖与呼吸综合征病毒GX1001株和GX1002株全基因组分子特征分析

Analysis of Genomic Characteristics of Porcine Reproductive and Respiratory Syndrome Virus GX1001 and GX1002 Strains Isolated in Guangxi Province

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