关键词: 庆大霉素和林可霉素注射液; 急性毒性; 溶血试验; 刺激试验; 过敏试验

Abstract: To evaluated the safety of Gentamicin and Lincomycin injection, by acute toxicity test, hemolysis test, local vascular, muscleand skin irritation tests and systemic allergy test in guinea, the safety of Gentamicin and Lincomycin injection was inspected. LD₅₀ of Gentamicin and Lincomycin injection by intramuscrular adminstration in mice was 4.989 mL/kg, hemolysis or coacervation in the red blood cells in rabbts did not occur for 0.1 to 0.5 mL of the injection in 4 h; Pathological changs such as hyperaemia,edema or hemorrhage were not examined in the veins where were injected intravenousiy and their surrounding tissues the hyperaemia ranged below 0.5 cm×1.0 cm on the intramuscular injection site, the ifference value between the maximum and the minimum order of reaction in quadriceps was zero， moreover the sum of them was less than 10;Double dose of the clinical doage of the injection was smeared on the rabbit skin, obvious pathological changes were not observed after the drug withdrawal for 1，24，48 and 72 h; Anaphylactic reaction was not observed in the guinea pigs after they were first sensibilized for 14 and 21 d. The results showed that, under this experimental condition, Gentamicin and Lincomycin injection was safe.

Key words: Gentamicin and Lincomycin injection; acute toxicity; hemolysis; irritation test; allergy test