犏牛主要组织相容性复合物DRA基因 (MHC-ClassⅡ-DRA)的分子特征分析

中国畜牧兽医

犏牛主要组织相容性复合物DRA基因 (MHC-ClassⅡ-DRA)的分子特征分析

蒲兰屏¹, 席冬梅¹, 樊月园¹, 苏八七², 陈学礼³, 李继中³, 邓卫东¹

1. 云南农业大学动物科学技术学院,云南昆明 650201;
2. 云南省怒江傈僳族自治州贡山独龙族怒族自治县农业和科学技术局,云南贡山 673500;
3. 云南省迪庆州畜牧兽医科技局,云南香格里拉 674400

收稿日期:2012-01-13 出版日期:2012-09-20 发布日期:2012-09-18
通讯作者: 邓卫东(1972－),男,陕西人,教授,博士,主要从事家畜遗传资源的分子评估研究。E-mail: dwdxdm@yahoo.com.cn E-mail:dwdxdm@yahoo.com.cn
作者简介:蒲兰屏(1985－),女,四川人,硕士生,主要从事抗病育种研究。
基金资助:
国家自然科学基金(31101640);云南省中青年学术和技术带头人后备人才项目(2008PY039)。

Analysis of the Molecular Characteristics of Chinese Yakow Major Histocompatibility Complex DRA Gene(MHC-ClassⅡ-DRA)

PU Lan-ping¹, XI Dong-mei¹, FAN Yue-yuan¹, SU Ba-qi², CHEN Xue-li³, LI Ji-zhong³, DENG Wei-dong¹

1. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
2. Agriculture Science and Technology Bureau, Gongshan Dulong and Nu Autonomous County, Nujiang Lisu Autonomous Prefecture of Yunan Province, Gongshan 673500, China;
3. Diqing Science and Animal Husbandry Bureay, Shangerila 674400, China

Received:2012-01-13 Online:2012-09-20 Published:2012-09-18

摘要/Abstract

摘要： 为探索犏牛的抗病特性,本试验运用1对引物首次扩增出了犏牛DRA基因的编码区全长,GenBank登录号为:JQ347519。通过NCBI开放阅读框寻找功能模块等生物信息学软件分析后结果表明,犏牛DRA基因编码区全长762 bp,编码253个氨基酸。对犏牛DRA基因编码产物进行了疏水性、信号肽、N-糖原区和二级结构预测,分析了犏牛DRA基因与其他物种的同源性,与黄牛相比,犏牛DRA基因表现出高度同源,同源性达到了99.0%。与MHC家族其他基因不同,DRA基因在所有的动物尤其是反刍动物中表现出高度保守,包括肽结合部位(PBS)、N-糖原区、α1、α2区等功能部位都呈现出高度保守。

关键词: 犏牛; MHC; DRA; 抗病性

Abstract: To explore the resistance traits of Chinese Yakow, in the present study, the Yakow coding region sequence of the DRA gene had been amplified by one pair primer and deposited into the GenBank database under accession JQ347519. According to the ORF FINDER from NCBI and other bioinformatics tools, the length of the coding region was 762 bp which encoded 253 amino acids. Moreover, the characteristics of hydrophobicity, signal peptide, N-linked glycosylation and the secondary structure were predicted. Analysis the homology with other species and compared with cattle, Yakow DRA (bogr×bola-DRA) sequence showed highest similarity (99.0%). Unlike other MHC molecules, the DRA gene showed highly conserved characteristics across all animals especially ruminants in functional positions, such as antigen binding at peptide binding site (PBS), N-linked glycosylation, α1 and α2 domains.

Key words: Chinese Yakow; MHC; DRA; resistance

中图分类号:

蒲兰屏, 席冬梅, 樊月园, 苏八七, 陈学礼, 李继中, 邓卫东. 犏牛主要组织相容性复合物DRA基因 (MHC-ClassⅡ-DRA)的分子特征分析[J]. 中国畜牧兽医.

PU Lan-ping, XI Dong-mei, FAN Yue-yuan, SU Ba-qi, CHEN Xue-li, LI Ji-zhong, DENG Wei-dong. Analysis of the Molecular Characteristics of Chinese Yakow Major Histocompatibility Complex DRA Gene(MHC-ClassⅡ-DRA)[J]. .

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