中药复方多糖对不同MHC B-LβⅡ基因型鸡淋巴细胞免疫信号分子表达的影响

doi:10.16431/j.cnki.1671-7236.2018.08.033

摘要/Abstract

摘要：

试验旨在探讨中药复方多糖对不同MHC B-LβⅡ基因型鸡免疫调节作用的影响。采用PCR-SSCP方法对500只试验鸡进行MHC B-LβⅡ基因分型，采集不同MHC B-LβⅡ基因型鸡的外周血淋巴细胞，分别加入终浓度为200、100、75、50、25、0 μg/mL的中药复方多糖共培养24 h，收集细胞，采用ELISA方法检测不同MHC B-LβⅡ基因型鸡淋巴细胞培养液中环磷酸腺苷（cAMP）、环磷酸鸟苷（cGMP）、钙离子（Ca²⁺）、一氧化氮（NO）及诱导型一氧化氮合成酶（iNOS）含量。结果显示，与对照组相比，中药复方多糖能显著提高不同MHC B-LβⅡ基因型鸡淋巴细胞培养上清液中cAMP、cGMP、Ca²⁺、NO和iNOS的含量（P<0.05）。中药复方多糖剂量为50 μg/mL时，AA、BC基因型鸡淋巴细胞培养上清液中cAMP、Ca²⁺、NO和iNOS的含量最高，BC基因型鸡淋巴细胞培养上清液中cGMP的含量最高；中药复方多糖剂量为75 μg/mL时，AA基因型鸡淋巴细胞培养上清液中cGMP的含量最高；中药复方多糖剂量为100 μg/mL时，BB基因型鸡淋巴细胞培养上清液中cAMP、cGMP、Ca²⁺、NO和iNOS的含量最高。本试验结果表明，中药复方多糖能不同程度地提高各MHC B-LβⅡ基因型鸡淋巴细胞培养上清液中cGMP、cAMP、NO、Ca²⁺和iNOS的含量，且不同MHC B-LβⅡ基因型鸡淋巴细胞最适中药复方多糖免疫调节剂量不同。

关键词: 中药复方多糖; MHC B-Lβ Ⅱ基因; 鸡淋巴细胞; 免疫信号分子

Abstract:

This study was aimed to explore the effect of compound Chinese herbal medicinal polysaccharides (cCHMPS) on immunomodulatory in different MHC B-LβⅡ genotype chickens.MHC B-LβⅡ genotype was analyzed by PCR-SSCP method in 500 chickens.Collected the blood from different MHC B-LβⅡ genotype chickens,isolated peripheral blood lymphocytes,and added cCHMPS with the final concentration of 200,100,75,50,25 and 0 μg/mL cocultured for 24 h.Then the lymphocyte supernatant cAMP,cGMP,Ca²⁺,NO and iNOS content were detected by ELISA.The results showed that cCHMPS could increase cAMP,cGMP,Ca²⁺,NO and iNOS levels in chicken lymphocyte supernatant compared with control group in each MHC B-LβⅡ genotype chickens.And when the concentration of cCHMPS was 50 μg/mL,cAMP,Ca²⁺,NO and iNOS levels of AA and BC genotypes chicken lymphocyte supernatant were higher than that of the same genotype chicken,cGMP level of BC genotype chicken lymphocyte supernatant was higher than that of the same genotype chicken;When cCHMPS concentration was 75 μg/mL,cGMP level of AA genotype chicken lymphocyte supernatant was higher than that of the same genotype chicken;When cCHMPS concentration was 100 μg/mL,cAMP,cGMP,Ca²⁺,NO and iNOS levels of BB genotype chicken lymphocyte of the supernatant were higher than that of the same genotype chicken.cCHMPS could increase cAMP,cGMP,Ca²⁺,NO and iNOS levels in different MHC B-LβⅡ genotypes chickens,and the cCHMPS optimum immunomodulatory doses were different in each MHC B-LβⅡ genotypes chickens.

Key words: compound Chinese herbal medicinal polysaccharides (cCHMPS); MHC B-LβⅡ gene; chicken lymphocyte; immune signal molecules

中图分类号:

S853.7

马昭, 商云霞, 陈洁, 刘晓婷, 梁小瑞, 朱晓庆, 谷新利. 中药复方多糖对不同MHC B-LβⅡ基因型鸡淋巴细胞免疫信号分子表达的影响[J]. 《中国畜牧兽医》, 2018, 45(8): 2312-2319.

MA Zhao, SHANG Yunxia, CHEN Jie, LIU Xiaoting, LIANG Xiaorui, ZHU Xiaoqing, GU Xinli. Effects of Compound Chinese Herbal Medicinal Polysaccharides on Immune Signal Molecules Expression in Different MHC B-LβⅡ Genotypes Chickens Lymphocyte[J]. , 2018, 45(8): 2312-2319.

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